Sequencing and Phylogenetic Analysis of the Salmonella Enterotoxin (stn) Gene of Salmonella spp. Isolated from Egyptian Broiler Breeder Chickens Farms
Azab, M., Zowail, M., A., N., Elsadek, G., Mohamed, S. (2019). Sequencing and Phylogenetic Analysis of the Salmonella Enterotoxin (stn) Gene of Salmonella spp. Isolated from Egyptian Broiler Breeder Chickens Farms. EKB Journal Management System, 11(3), 139-147. doi: 10.21608/eajbsc.2019.70775
Mona S. Azab; Mohamed E. M. Zowail; Nassif S. A.; Ghada M. Elsadek; Safwa Z. Mohamed. "Sequencing and Phylogenetic Analysis of the Salmonella Enterotoxin (stn) Gene of Salmonella spp. Isolated from Egyptian Broiler Breeder Chickens Farms". EKB Journal Management System, 11, 3, 2019, 139-147. doi: 10.21608/eajbsc.2019.70775
Azab, M., Zowail, M., A., N., Elsadek, G., Mohamed, S. (2019). 'Sequencing and Phylogenetic Analysis of the Salmonella Enterotoxin (stn) Gene of Salmonella spp. Isolated from Egyptian Broiler Breeder Chickens Farms', EKB Journal Management System, 11(3), pp. 139-147. doi: 10.21608/eajbsc.2019.70775
Azab, M., Zowail, M., A., N., Elsadek, G., Mohamed, S. Sequencing and Phylogenetic Analysis of the Salmonella Enterotoxin (stn) Gene of Salmonella spp. Isolated from Egyptian Broiler Breeder Chickens Farms. EKB Journal Management System, 2019; 11(3): 139-147. doi: 10.21608/eajbsc.2019.70775

Sequencing and Phylogenetic Analysis of the Salmonella Enterotoxin (stn) Gene of Salmonella spp. Isolated from Egyptian Broiler Breeder Chickens Farms

Egyptian Academic Journal of Biological Sciences. C, Physiology and Molecular Biology
Article 13, Volume 11, Issue 3, December 2019, Page 139-147  XML PDF (662.59 K)
Document Type: Original Article
DOI: 10.21608/eajbsc.2019.70775
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Authors
Mona S. Azab1; Mohamed E. M. Zowail2; Nassif S. A.3; Ghada M. Elsadek3; Safwa Z. Mohamed3
11-Department of Biology, Faculty of Science, Jouf University, Skaka, KSA. 2-Department of Zoology, Faculty of Science, Benha University, Egypt
2Department of Zoology, Faculty of Science, Benha University, Egypt
3Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt
Abstract
    Salmonella is a member of Enterobacteriaceae family that found to be pathogenic to domestic and wild animals and humans. Salmonellae were isolated from three distinct governorates, Giza, Monofia and Qaluobia from broiler breeder chicken farms. Molecular characterization of the Salmonella isolates using polymerase chain reaction (PCR) assay as well as Sequencing and phylogenetic analysis of PCR products were conducted to distinguish the collected Salmonellae species.
The nucleotide sequence of 617 bp PCR products representing the amplified fragment of stn gene of seven isolates of Salmonella enteritidis has been sequenced. Furthermore, the nucleotide sequence was submitted to the gene bank.  The obtained sequences were blasted with the highly similar sequences and the multiple sequence alignments were conducted. Neighbor-joining tree was constructed for the Egyptian Salmonella isolates against 30 Salmonella spp. from the Gene bank database representing maximum similarity with stn gene when subjected to multiple sequence alignment, and the phylogenetic tree was constructed based on the comparative analysis of related sequences at the nucleotide level.
Keywords
Salmonella; PCR; stn gene; Sequencing
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