Glutathione and selenium as chemopreventive agents on experimentally induced hamster buccal pouch epithelial dysplasia | ||||
| Egyptian Dental Journal | ||||
| Article 12, Volume 65, Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), January 2019, Page 331-342 PDF (2.69 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/edj.2015.71421 | ||||
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| Authors | ||||
| Kamal Abd Elrahman Kamal1; Amr Saad AbdoAlwahab2; Abd Elnasser Abd El mola Esmail1 | ||||
| 1Lecturer of Oral Pathology - Faculty of Dentistry - Al-Azhar University | ||||
| 2Assistant Professor of Oral Biology - Faculty of Dentistry - Al-Azhar University | ||||
| Abstract | ||||
| The aim of the present study was directed to investigate the effect of Glutathione (GSH) and Selenium (Se) as chemopreventive agents in experimentally induced hamster buccal pouch (HBP) epithelial dysplasia. Material and methods: Twenty five golden Syrian male hamsters were used as experimental animals and divided into five groups (G1, G2, G3, G4 and G5): G1: 5 animals were left untreated. G2: 5 animals, their right buccal pouches were painted with 0.5% 7, 12 dimethylbenz [a] anthracene (DMBA) 3 times a week for 8 weeks. G3: 5 animals were received GSH one week before, as well as during the application of DMPA for 8 weeks. G4: 5 animals were received Se one week before, as well as during the application of DMPA for 8 weeks. G5: 5 animals were received GSH and Se one week before, as well as during the application of DMPA for 8 weeks. After termination of the experiment, all animals were sacrificed, and the buccal mucosa was excised and preparation in order to be examined histologically and immunohistochemically, then statistical analysis based on these examinations was done Results: Regard to expression of Bcl-2, G2 had recorded the highest mean area percentage (44.25%), while G1 had recorded the lowest mean area percentage (6.73%) and there was high significant difference between G1,G2, G3, G4 and G5 where P value was <0.001, while in regard to expression of Bak, G1 had recorded the highest mean area percentage (41.02%), while G2 had recorded the lowest mean area percentage (19.79%) and there was significant difference between G1, G2, G3, G4 and G5 where P value was 0.019. Conclusion: GSH and Se are considered as promising chemotherapeutic agents in prevention of induced HBP epithelial dysplasia and proved important role in apoptosis and proliferation throughout the process of carcinogenesis. | ||||
| Keywords | ||||
| HBP carcinogensis; GSH; Se; Bcl2; Bak | ||||
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