SOME FACTORS AFFECTING FERTILIZNIG CAPACITY OF FROZEN GOAT SEMEN | ||||
| Journal of Animal and Poultry Production | ||||
| Article 1, Volume 4, Issue 10, October 2013, Page 561-570 PDF (624.18 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jappmu.2013.71534 | ||||
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| Author | ||||
| A. A. Sallam* | ||||
| Animal Production Research Institute, Agricultural Research Center, Dokki, Giza, Egypt | ||||
| Abstract | ||||
| The present study aimed to study some factors affecting the successful artificial insemination (AI) including optimal level of egg yolk and dilution rate of extender, semen collection month and AI procedures of crossbred buck semen. Semen was collected from10 fertile goat bucks (1/2 Damascus x 1/2 Baladi) throughout all months of the year. ُEjaculates with mass motility ≥80% were pooled, diluted with Tris-citric acid-glucose-glycerol extender containing different levels of egg yolk (2.5, 5.0, 7.5, 10.0, 15.0 and 20%) or diluted with different rates (1:4, 1:5, 1:8, 1:10 and 1:16) with the best level of egg yolk (2.5%), then semen was frozen with the best level of egg yolk (2.5%) and dilution rate (1:5) during all months of the year for all treatments. Sperm motility was evaluated and recovery rate was calculated for each treatment. Fertility trail was conducted using sixty four synchronized does by Estrumate. Does were inseminated (intra-vaginal vs. intra-uterine) with inseminated dose (100 vs. 200 x 106) after 48 or 52 h of Estrumate-injection. Results revealed that sperm motility percentage was the highest (P<0.05) in post-thawed semen diluted with Tris-extender containing 2.5% egg yolk (54.8%) and at a dilution rate of 1:5 (54.5%). Post-thaw sperm motility percentage was the highest during autumn months, in particular, in November and the lowest during summer months (June-Aug.). Fertility rate (FR) was the highest (P<0.05) of does intra-uterine inseminated with high dose of insemination after 48 h of Estrumate-injection (62.5%), while the lowest FR was recorded for the control does (43.75%). Does inseminated intra-uterine with low dose after 48 h of Estrumate-injection and those inseminated intra-uterine with high dose after 52 h of Estrumate-injection had moderate rates of fertility, being 50.0 and 56.25%, respectively. In conclusion, dilution of buck semen with Tris-based extender containing 2.5% egg yolk at a rate of 1:5 and frozen in pellets form will be more efficient in maintaining post-thaw sperm motility. The best season of collection and freezing goat semen is autumn. Intra-uterine insemination with high dose of motile spermatozoa (200 x 106) could yield acceptable fertility rates of goat does. | ||||
| Keywords | ||||
| Goat; egg yolk; dilution rate; season; intra-uterine insemination; fertility | ||||
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