INJECTION EFFECT OF GIBBERELLIC AND/ OR BORIC ACID TO RABBITS ON RESPONSE OF SPERMATOZOA TO HYPO-OSMOTIC SWELLING TEST | ||||
| Journal of Animal and Poultry Production | ||||
| Article 2, Volume 4, Issue 10, October 2013, Page 571-581 PDF (671.09 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jappmu.2013.71540 | ||||
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| Authors | ||||
| Fadila M. Easa; Amal M. Hekal; T. S.T. Seleem | ||||
| Department of Rabbit Breeding Research, Animal Production Research Institute, Agricultural Research Center, Dokki, Giza, Egypt. | ||||
| Abstract | ||||
| A total number of forty eight sexually mature New-Zealand White (NZW) rabbit bucks of nine months age were used in this experiment. The experiment was designed to evaluate grade and percentages of progressive sperm motility, in addition to percentages of spermatozoa with swelling heads and coiled tails ejaculated by bucks injected with Gibberellic and/ or Boric acid as responded to hypo-osmotic solution, during incubation at 37 oC for up to 60 minutes. Animals were divided into four experimental comparable groups (12 in each). Rabbits in 1st group were subcutaneously injected with 1 ml saline solution (NaCL) and served as a control group, while the rabbits in 2nd, 3rd and 4th groups were injected subcutaneously with 1 mg Gibberellic acid, 2.5 mg Boric acid and mixture of 1 mg Gibberellic and 2.5 mg Boric acids, respectively. Rabbit injection started 6 weeks before semen collection and during experimental period, weekly. The results obtained from this study indicated that, sperm motility in solutions with different osmolarities (300 or 100 mOsmol/ L) were significantly (P ≤ 0.01) higher, while the percentages of spermatozoa with coiled tails or swollen heads were significantly (P ≤ 0.01) lower at level of 300 mOsmol/ L (normal osmolarity) than 100 mOsmol/ L (osmotic stress), during incubation at 37 oC for up to 60 minutes in all studied rabbit semen groups. Advancement of incubation time up to 60 minutes of rabbit semen in solutions with different osmolarities decreased and increased significantly (P ≤ 0.01) {grade & advanced sperm motility} and {spermatozoa with coiled tails & swollen heads}, respectively, of all studied rabbit semen groups. Values of each of progressive sperm grades & percentages and percentages of spermatozoa with coiled tails or swollen heads in both osmolarities used (300 and 100 mOsmol/ L) were significantly (P ≤ 0.01) arranged descendingly as recorded by semen of rabbit injected with mixture of Gibberellic + Boric acids followed by Boric acid, Gibberellic acid then saline solution (NaCL) control, respectively. It be concluded that, injecting rabbit bucks with Gibberellic and Boric acids improved their semen quality, during semen conservation at incubation condition. | ||||
| Keywords | ||||
| Rabbit; Semen; Gibberellic; Boric; incubation; HOS-test | ||||
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