Speculation of the Cytotoxic Effect of Cisplatin Either Necrosis or Apoptosis and the Possible Antagonist Protective Role of Acetyl L-Carnitine, In vitro Study
Taha, R., Said, R. (2019). Speculation of the Cytotoxic Effect of Cisplatin Either Necrosis or Apoptosis and the Possible Antagonist Protective Role of Acetyl L-Carnitine, In vitro Study. EKB Journal Management System, 65(Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)), 1297-1309. doi: 10.21608/edj.2019.72554
Rasha Mohamed Taha; Rania Hanafi Mahmoud Said. "Speculation of the Cytotoxic Effect of Cisplatin Either Necrosis or Apoptosis and the Possible Antagonist Protective Role of Acetyl L-Carnitine, In vitro Study". EKB Journal Management System, 65, Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), 2019, 1297-1309. doi: 10.21608/edj.2019.72554
Taha, R., Said, R. (2019). 'Speculation of the Cytotoxic Effect of Cisplatin Either Necrosis or Apoptosis and the Possible Antagonist Protective Role of Acetyl L-Carnitine, In vitro Study', EKB Journal Management System, 65(Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)), pp. 1297-1309. doi: 10.21608/edj.2019.72554
Taha, R., Said, R. Speculation of the Cytotoxic Effect of Cisplatin Either Necrosis or Apoptosis and the Possible Antagonist Protective Role of Acetyl L-Carnitine, In vitro Study. EKB Journal Management System, 2019; 65(Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)): 1297-1309. doi: 10.21608/edj.2019.72554

Speculation of the Cytotoxic Effect of Cisplatin Either Necrosis or Apoptosis and the Possible Antagonist Protective Role of Acetyl L-Carnitine, In vitro Study

Egyptian Dental Journal
Article 15, Volume 65, Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), April 2019, Page 1297-1309  XML PDF (3.87 MB)
Document Type: Original Article
DOI: 10.21608/edj.2019.72554
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Authors
Rasha Mohamed Taha1; Rania Hanafi Mahmoud Said2
1Lecturer in Oral Biology Department, Faculty of Dentistry, Suez Canal University
2Assistant professor in Oral Pathology Department, Faculty of Dentistry, Suez Canal University
Abstract
Background: Cisplatin is one of mostly used chemotherapeutic drugs. Eventhough, cisplatin drawbacks limit its use. Acetyl L- Carnitine is a neuro-protective and anti-oxidant agents as well as it has anti-apoptotic properties.
Aim of the study: this study was designed to evaluate the mechanism of Cisplatin to influence tissue in the pathway of apoptosis or the pathway of necrosis and the protective role of Acetyl-L-Carnitine against cytotoxicity induced by Cisplatin in the dental pulp.
Material and methods: Total of 30 male albino rats (250-300 grams) were divided equally into three groups where saline was given to the control group (Group I), Cisplatin was injected into group II the (Cisplatin Group), and L- Carnitine was given to the Group III (L- Carnitine group + Cisplatin) before the injection of Cisplatin. After sacrificing the rats one week later, the extraction and preparation of their jaws were carried out in order to examine their dental pulp histologically histologically and immunohistochemically.
Results: The light microscopic results showed degeneration in the dental pulp tissue of group II animals represented by cytoplasmic vacuolization, idiopathic calcification, hyaline and fatty degeneration. While group III showed normal dental pulp tissues with dilated blood vessels. Immunohistochemical examination showed significant differences in group II when compared to control group for both Bax (p=0.0002) and TNF-α (p=0.0029). No significant differences appeared in group III when compared to control group for both Bax and TNF-α. Significant differences were evident in comparison between group II and group III for both Bax (p=0.0015) and TNF-α (p=0.000001).
Conclusion: Cisplatin has a devastating effect on dental pulp tissues via both the apoptosis and necrosis pathways. L-Carnitine had a protective effect against the cytotoxicity of Cisplatin.
Keywords
Cisplatin; L-carnitine; BAX protein; Tumour Necrosing Factor
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