Effect of chitosan on resin-dentin interface durability: A 2 year in-vitro study | ||||
| Egyptian Dental Journal | ||||
| Article 52, Volume 65, Issue 3 - July (Fixed Prosthodontics, Dental Materials, Conservative Dentistry & Endodontics), July 2019, Page 2955-2965 PDF (506.38 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/edj.2019.72691 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| Ahmed Zidan | ||||
| Lecturer, Dental Materials Department, Faculty of Dentistry, October University for Modern Sciences and Arts (MSA). | ||||
| Abstract | ||||
| Aim: The aim of the current study was to evaluate immediate and long-term bonding effectiveness of chitosan-treated dentin, bonded using 2-step etch-and-rinse dental adhesive, after 2 years of ageing. Materials & Methods: Microtensile bond strength test and nanoleakage examination of resin-dentin interfaces, created by Adper Single Bond 2 with or without chitosan pre-treatment, were performed after 24 h, 12 mos and 24 mos of water storage. Stiffness of demineralized dentin sticks treated with chitosan for 1 or 10 min was evaluated. Matrix metalloproteinases (MMPs) activity was also assessed by measuring the amount of hydroxyproline release, indicating collagen degradation by MMPs, after chitosan treatment for 1 or 10 min. Data were collected, tabulated and statistically analysed. Results: Chitosan treatment of acid-etched dentin, before adhesive application, resulted in microtensile bond strength values which are significantly higher (40.7± 1.7 MPa and 37.2±1.9 MPa respectively) and nanoleakage values (56.5±3.8% and 62±3.6 % respectively) that are significantly lower than the untreated control group at 12 mos and 24 mos storage periods (P ≤ 0.05). The stiffness of demineralized dentin increased significantly after 1 min chitosan application (12.6±1.8 MPa) and further increased after 10 min chitosan application (22.4± 2.2 MPa). Hydroxyproline released decreased significantly (P-value <0.001) when completely demineralized dentin was treated with chitosan for 1 min (1.1 µg/mg dentin) with further significant decrease when chitosan was used for 10 min (0.8 µg/mg dentin). Conclusion: Chitosan treatment of acid-etched dentin, before adhesive application, was effective in improving durability of resin-dentin bonded interfaces. Clinical Significance: Despite of the great advances that have occurred in the field of adhesive dentistry, still, biodegradation of resin-dentin bonds over time continues to jeopardize the durability and success of resin composite restorations. | ||||
| Keywords | ||||
| Chitosan; etch-and-rinse adhesive; Dentin; microtensile bond strength; Nanoleakage; Stiffness; hydroxyproline release | ||||
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