Salivary Antisense Non-Coding RNA In the INK4 Locus & Maternal Expressed Gene 3 as early biomarkers in Detection of Malignant Transformation of Oral Lichen Planus
Ghalwash, D., Shaker, O., Elshiwy, Y., Radwan, N. (2019). Salivary Antisense Non-Coding RNA In the INK4 Locus & Maternal Expressed Gene 3 as early biomarkers in Detection of Malignant Transformation of Oral Lichen Planus. EKB Journal Management System, 65(Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)), 2419-2424. doi: 10.21608/edj.2019.72893
Dalia Ghalwash; Olfat G. Shaker; Yasmine Elshiwy; Noha A. Radwan. "Salivary Antisense Non-Coding RNA In the INK4 Locus & Maternal Expressed Gene 3 as early biomarkers in Detection of Malignant Transformation of Oral Lichen Planus". EKB Journal Management System, 65, Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), 2019, 2419-2424. doi: 10.21608/edj.2019.72893
Ghalwash, D., Shaker, O., Elshiwy, Y., Radwan, N. (2019). 'Salivary Antisense Non-Coding RNA In the INK4 Locus & Maternal Expressed Gene 3 as early biomarkers in Detection of Malignant Transformation of Oral Lichen Planus', EKB Journal Management System, 65(Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)), pp. 2419-2424. doi: 10.21608/edj.2019.72893
Ghalwash, D., Shaker, O., Elshiwy, Y., Radwan, N. Salivary Antisense Non-Coding RNA In the INK4 Locus & Maternal Expressed Gene 3 as early biomarkers in Detection of Malignant Transformation of Oral Lichen Planus. EKB Journal Management System, 2019; 65(Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)): 2419-2424. doi: 10.21608/edj.2019.72893

Salivary Antisense Non-Coding RNA In the INK4 Locus & Maternal Expressed Gene 3 as early biomarkers in Detection of Malignant Transformation of Oral Lichen Planus

Egyptian Dental Journal
Article 19, Volume 65, Issue 3 - July (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), July 2019, Page 2419-2424  XML PDF (522.34 K)
Document Type: Original Article
DOI: 10.21608/edj.2019.72893
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Authors
Dalia Ghalwash1; Olfat G. Shaker2; Yasmine Elshiwy3; Noha A. Radwan4
1Associate Professor of Oral Medicine and Periodontology, Faculty of Dentistry, The British University in Egypt (BUE), Cairo, Egypt
2Professor of Biochemistry, Faculty of Medicine, Cairo University, Cairo, Egypt
3Associate Professor of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt
4Lecturer of Clinical and Chemical Pathology, Faculty of Medicine, Cairo University, Cairo, Egypt
Abstract
Oral lichen planus (OLP) is a potentially malignant lesion. It is crucial to properly identify patients at risk of malignant transformation. LncRNA antisense non-coding RNA in the INK4 locus (ANRIL) has been known to advance tumor progression. Another type of lncRNAs is maternal expressed gene (MEG3) which is a tumor suppressor gene that can inhibit the advancement of malignancy. The goal of this study was to outline the relative fold expression of ANRIL and MEG3 in OLP and oral squamous cell carcinoma (OSCC). The study included 20 histopathologically diagnosed OLP patients and 10 patients with OSCC. The relative fold expression of ANRIL and MEG3 in the OLP group was compared to OSCC group using qRT-PCR. The results showed that the relative fold expression of ANRIL was significantly increase in the OSCC compared to OLP group (p<0.01). ROC analysis showed that with cut-off value of 4.1 relative fold expression of ANRIL with sensitivity 0.8, specificity 0.7 and AUC 0.85. The decrease in MEG3 was insignificant. ANRIL relative fold expression could perform an essential part in OSCC progression, and could assist in the future as a potential biomarker for malignant transformation of OLP.
Keywords
LncRNAs – oral squamous cell carcinoma – qRT-PCR – salivary sample; ROC curve
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