Production of Salinity Tolerant Nile Tilapia, Oreochromis niloticus through Introducing Foreign DNA into Fish Gonads | ||||
Journal of Animal, Poultry & Fish Production | ||||
Article 1, Volume 2, Issue 1, December 2014, Page 1-10 PDF (1.98 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/japfp.2014.7430 | ||||
View on SCiNiTO | ||||
Authors | ||||
El-Zaeem Y.1; H. Khouriaba2; M. El-Sherif2; M. Shahin3 | ||||
1Animal and Fish Production Dept., Faculty of Agriculture, (Saba-Bacha), Alexandria University, Alexandria, Egypt. | ||||
2Animal Production and Fish Resources Dept., Faculty of Agriculture, Suez Canal University, 41522 Ismailia, Egypt | ||||
3Animal Production and Fish Resources Dept., Faculty of Agriculture, Suez Canal University, 41522 Ismailia, Egyp | ||||
Abstract | ||||
This study was conducted to produce a salinity tolerant Nile tilapia, Oreochromis niloticus through genetically modified breeding by introducing a fragmented purified DNA isolated from Artemia, Artemia salina into the gonads. Two groups of adult fish (16 females and 8 males) were chosen to be injected with the foreign DNA into their gonads using a hypodermic needle with two different concentrations (10 μg and 5 μg /0.1 ml/fish), besides the control group (4 males and 8 females) carried out. Post-hatching fry which produced from each treatment of DNA were collected and weighed then transferred separately to glass. Two salinity levels were used to rear the hatching fry during the present study- 20 ppt (equivalent to half the sea salinity level) and 40 ppt (equivalent to the sea salinity level) - beside the freshwater as a control. The results showed a significant improvement (P≤0.05) in most of the growth performance parameters of genetically modified O. niloticus treated with 10 μg of Artemia DNA compared to the lowest dose of 5 μg of DNA and the control fish reared at 20 ppt of salinity. The results also showed that, the number of amplified bands detected varied, depending on the primers and DNA treatment. Highly genetic polymorphic percentage ranged from (8.00 to 71.79%) with an average of 39.05% using different random primers. The results of the present work suggested that, hyper-saline genetically modified O. niloticus with higher growth rate can be produced using a feasible and fast methodology. | ||||
Keywords | ||||
Nile tilapia; Oreochromis niloticus; genetically modified fish; salinity tolerance; Growth performance | ||||
Statistics Article View: 146 PDF Download: 351 |
||||