Efficiency of Photodynamic Therapy and Avastin in Treatment of Corneal Neovascularization in Rabbit's Eye
El gohary, M., Abdelkawi, S., Hassan, A., Saif, A., El Shinawy, A. (2017). Efficiency of Photodynamic Therapy and Avastin in Treatment of Corneal Neovascularization in Rabbit's Eye. EKB Journal Management System, 18(1), 46-55. doi: 10.21608/ejbbe.2018.2840.1014
Mohamed Ismail El gohary; Salwa Ahmed Abdelkawi; Aziza Ahmed Hassan; Ahmed Tamer Saif; Ayman Salah El Shinawy. "Efficiency of Photodynamic Therapy and Avastin in Treatment of Corneal Neovascularization in Rabbit's Eye". EKB Journal Management System, 18, 1, 2017, 46-55. doi: 10.21608/ejbbe.2018.2840.1014
El gohary, M., Abdelkawi, S., Hassan, A., Saif, A., El Shinawy, A. (2017). 'Efficiency of Photodynamic Therapy and Avastin in Treatment of Corneal Neovascularization in Rabbit's Eye', EKB Journal Management System, 18(1), pp. 46-55. doi: 10.21608/ejbbe.2018.2840.1014
El gohary, M., Abdelkawi, S., Hassan, A., Saif, A., El Shinawy, A. Efficiency of Photodynamic Therapy and Avastin in Treatment of Corneal Neovascularization in Rabbit's Eye. EKB Journal Management System, 2017; 18(1): 46-55. doi: 10.21608/ejbbe.2018.2840.1014

Efficiency of Photodynamic Therapy and Avastin in Treatment of Corneal Neovascularization in Rabbit's Eye

Egyptian Journal of Biomedical Engineering and Biophysics
Article 5, Volume 18, Issue 1, 2017, Page 46-55  XML PDF (1.25 MB)
Document Type: Original Article
DOI: 10.21608/ejbbe.2018.2840.1014
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Authors
Mohamed Ismail El goharyorcid 1; Salwa Ahmed Abdelkawi2; Aziza Ahmed Hassan3; Ahmed Tamer Saif4; Ayman Salah El Shinawy email 1
1Physics Dpt., faculty of science (boys), Al azhar University
2Department of Vision Science, Biophysics and Laser Science Unit, Research Institute of Ophthalmology
3Ophthalmic Unit, National Institute for Laser Enhanced Science, Cairo University
4Ophthalmology Department, Fayoum University
Abstract
The aim of the present work is to evaluate the effect of photodynamic therapy (PDT) by using argon laser with rose bengal (Herbs) as a photosensitizer when compared to the Avastin for treatment of corneal neovascularization (CNV) in rabbits. Thirty-Nine New Zealand male rabbits aged 2.5 months and weighing 2.5-3 kg are selected from the animal house of research institute of ophthalmology, Giza, Egypt. Three rabbits are used as control, and 36 rabbits are subjected to corneal neovascularization (CNV) induced by 3 silk sutures at mid-stromal depth approximately 1 mm from the limbus. After four days, sutures are removed and slit lamp examinations are performed to determine the area of CNV. Rabbits with CNV are divided into 3 groups: (1) The first group left without any treatment(n=12); (2) The second group (n=12 rabbits) were generally anesthetized and rose bengal(C20H4Cl4I4O5) with a dose of 50 mg/Kg was administered by slow intravenous injection in the marginal ear vein. After 15 minutes, rabbit’s eyes were exposed to argon laser(Quantel-Medical, Vitra, France) in a pulsed mode at 532 nm, spot size of 50 μm, pulse duration of 0.2 ms and a power of 150-200 mW/cm 2.; (3) The third group are treated with subconjunctival injection of 25 mg Avastin (n=12). The rabbits are followed up for 4 weeks and 3 rabbits are selected and sacrificed weekly from each group. The corneas are isolated for determination of protein content, SDS-poly acrylamide gel electrophoresis, total antioxidant capacity (TAC) and total oxidative capacity (TOC). Final results show that avastin is almost as effective as photodynamic therapy.
Keywords
Corneal neovascularization (CNV); Photodynamic therapy (PDT); argon laser; Avastin
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