IN VITRO EVALUATION OF MECHANICAL AND STRUCTURAL PROPERTIES OF LEUKOCYTE-PLATELET-RICH FIBRIN AND PLATELET-RICH FIBRIN MATRIX | ||||
| Egyptian Dental Journal | ||||
| Article 13, Volume 63, Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), April 2017, Page 1531-1539 PDF (1.45 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/edj.2017.74550 | ||||
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| Authors | ||||
| Reham Lotfy Aggour1; Hadeel A. Sabry2; Gihan Shehatah Hassan3 | ||||
| 1Lecturer of Oral Medicine, Periodontology, Oral Diagnosis and Oral Radiology, Faculty of Dentistry, October 6 University | ||||
| 2Associate professor of Dental Biomaterials, Faculty of Dentistry, October 6 University | ||||
| 3Lecturer of Oral Biology, Faculty of Dentistry, Tanta University | ||||
| Abstract | ||||
| Objectives: The aim of this study was to investigate the mechanical properties as well as the structural analysis of autologous platelet-rich fibrin matrix (PRFM) as compared to autologous leukocyte-platelet-rich fibrin (L-PRF). Materials& Methods: Sixty three cubic centimeters (cc) venous blood was obtained from 10 male volunteers. Twenty seven cc were used to prepare 3 specimens of L-PRF (group 1), twenty seven cc were used to get 3 specimens of PRFM (group 2) and nine cc for whole blood analysis. After centrifugation, blood analyses were performed on the residual plasma after collecting L-PRF and PRFM. The L-PRF and PRFM membranes were processed for examination by light microscopy and transmission electron microscopy (TEM) and their mechanical properties were measured by a universal testing machine. Results:Tensile strength and maximum tensile strain of L-PRF group was significantly higher than PRFM group (p < 0.01). PRFM group was significantly stiffer than L-PRF group (p < 0.01). Light microscopy revealed that the platelets were less but more equally distributed in the PRFM than L-PRF. The border between the cellular components and the fibrin network appeared thicker in the PRFM samples than in the L-PRF samples and shows a highly organized network with continuous integrity. TEM showed that both membranes contained two components: a fibrillar material similar to fibrin filaments, and a cellular component that contains human platelet cells. TEM analysis demonstrated that PRFM membrane had more nonactivated platelets. Conclusion: The present study shows that the structural and mechanical properties of PRFM may fit characteristics desirable for GTR procedures more than that of L-PRF. | ||||
| Keywords | ||||
| Mechanical Properties; platelet-rich fibrin matrix; leukocyte-platelet-rich fibrin | ||||
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