EXPRESSION OF MMP-1 AND MMP-9 IN LOCALIZED AGGRESSIVE PERIODONTITIS PATIENTS BEFORE AND AFTER TREATMENT: A CLINICAL AND IMMUNOHISTOCHEMICAL STUDY | ||||
| Egyptian Dental Journal | ||||
| Article 21, Volume 63, Issue 1 - January (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), January 2017, Page 667-684 PDF (5.12 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/edj.2017.75015 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Hala H. Hazzaa1; Eman A. Abo Hager2 | ||||
| 1Assistant Professor of Oral Medicine, Periodontology, Diagnosis and Radiology. . Faculty of Dental Medicine for Girls. Al Azhar University. | ||||
| 2Assistant Professor of Oral and Dental Pathology. Faculty of Dental Medicine for Girls. Al Azhar University. | ||||
| Abstract | ||||
| Aim: This study aimed to clinically investigate the efficacy of β-glucan supplementation to non-surgical periodontal therapy in localized aggressive periodontitis (LAP) patients. In parallel, immunohistochemical detection of matrix metalloproteinase-1 (MMP-1) and MMP-9 expression levels has undergone to determine the subcellular changes behind the clinical status and reliability of each antibody in predicting the periodontal condition. Method: 40 subjects suffering from LAP were randomly and equally assigned to receive scaling and root planning (SRP); either with placebo pills (Group I) or β-glucan (100 mg/ once a day) (Group II), for 40 days. Subjects were clinically monitored on day 0 and day 91. Gingival samples were harvested from hopeless teeth sites to be investigated histologically and immunohistochemically using matrix metalloproteinase (MMP)-1 and 9 antibodies form each participating patient; at the same time intervals. Results: The experimental intervention showed a greater mean of probing pocket depth reduction (p=0.1128), with significant gain in clinical attachment (p=0.0180) and reduction of gingival inflammation (p=0.0207) compared to the control group at the end of the study. Furthermore, the experimental protocol was able to achieve better modulating effects on the levels of MMP-1 and MMP-9 compared to control therapy, along with enhancing protective healing patterns. MMP-9 was more sensitive indicator of the periodontal condition compared to MMP-1. Conclusions: The curre nt study demonstrated that the non-surgical treatment of LAP is markedly improved clinically by the adjunctive use of β-glucan, accompanied by a trend for modulation of the cytokine profile in gingival tissue samples with better healing events. The results also suggest that the expression of MMP-9 may be a precise indicator of periodontal disease activity. | ||||
| Keywords | ||||
| β-glucan; localized aggressive periodontitis; matrix metalloproteinases; randomized double blind clinical trials | ||||
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