ANTIFUNGAL ACTIVITY OF PLANT EXTRACTS, ESSENTIAL OILS, AND MICROBIAL CULTURE FILTRATES AGAINST Botrytis cinerea In-vitro | ||||
| Journal of Plant Protection and Pathology | ||||
| Article 13, Volume 6, Issue 9, September 2015, Page 1297-1311 PDF (253.75 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jppp.2015.75199 | ||||
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| Authors | ||||
| Y. M. Shabana1; M. S.S. El-Boray2; M. F.M. Mustafa2; G. A.M. Al-Juboori3 | ||||
| 1Plant Pathology Department, Faculty of Agriculture, Mansura University, Egypt | ||||
| 2Pomology Department, Faculty of Agriculture, Mansura University, Egypt | ||||
| 3Pomology Department, Faculty of Agriculture, Mansura University, Egypt Horticulture Department, Fac. Agric. Tech., SulaymaniaPolytechnic Univ., Iraq | ||||
| Abstract | ||||
| The effect of using plant extracts and oils of plant origin on the colony growth of Botrytis cinerea, the causal agent of gray mold of fruits and vegetables was studied five plant extracts (pomegranate peel, cloves, neem, cinnamon and thyme) at three concentrations(0, 15, 30 and 45%; for the aqueous extracts and 0, 6, 9 and 15%; for the extracts of methyl alcohol and ethyl estate) were used Essential oils of colves, neem, cinnamon, and thyme at three concentrations (0, 1, 1,5 and 2%) as well as culture filtrates of Trichodermaharzianum, T. hamatum and Bacillus subtilis at three concentrations (0, 15, 30 and 45%) were also tested. The fungicide, Topsin M-70WP at the recommended concentration (80g/100L water) was used as a check for comparison. In general, for the concentrations 1 and 2, there were no significant differences among the three methods of extraction (water, methyl alcohol, and ethyl acetate) also givenaqueous fraction of crude plant extracts significant reduction in the linear growth of B. cinerea ranged from (57.78 to 96.67%) Pomegranate peel andneem aqueous extracts caused the highest growth inhibition at 45% concentration 96.67 and 92.22% respectively with no significant difference from the fungicide effect at same concentration. While, given methyl alcohol and ethyl acetate All plant extracts produced significant growth inhibition of B. cinerea 7 days post inoculation in comparison with the untreated control. both fractions (methyl alcohol and ethyl acetate), it was found that all plant extracts tested at 15%, except thyme extract, caused more than 89% growth inhibition to the pathogen with no significant differences from the chemical fungicide, Topsin M-70WP. Essential oils given tested caused significant suppression of the pathogen growth, 7 days post inoculation in comparison with the untreated control. The growth inhibition of the pathogen increased with the increase of the plant oil concentration. The chemical fungicide, Topsin M-70WP, had the highest inhibitory effect against the pathogen’s growth, which was followed by cloves and neem essential oils when tested at 2% concentration. While,all culture filtrates tested caused Record significant inhibition of the pathogen growth, 7 days after inoculation in comparison with the untreated control.. The bacterial culture filtrate of B. subtilis had the highest inhibitory effect against the pathogen’s growth, with no significant difference from the chemical fungicide, Topsin M-70WP. | ||||
| Keywords | ||||
| Plant extracts; plant oils; microbial culture filtrate; Botrytis cinerea; biological control; antifungal activity; grape | ||||
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