BIOCHEMICAL GENETICS OF RESISTANCE AND CROSS RESISTANCE OF Spodoptera littoralis (Boisd.) PROGNEY TO CHLORPYRIFOS USING DEVELOPED REARING TECHNIQUE | ||||
| Journal of Plant Protection and Pathology | ||||
| Article 9, Volume 6, Issue 11, November 2015, Page 1555-1566 PDF (249.46 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jppp.2015.75428 | ||||
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| Authors | ||||
| A. H. Mohanna; H. M. El-Sharkawy | ||||
| Dept. of plant production ,Faculty of Technology & Development, Zagazig Uni., Egypt | ||||
| Abstract | ||||
| In laboratory tests, a unique technique was carried out to distinguish and obtain developed strains originated from separately reared Spodoptera littoralis (Boisd.) egg- masses ( field and laboratory strains); traditional susceptible strain (Tss) from laboratory strain, and traditional resistant strain ( Trs). from field. Two other strains were obtained in laboratory; the first developed traditional susceptible strain ( dTss.) was originated from the high susceptible larvae of Tss , and was subjected to relaxation for four successive generations. while the second, developed traditional resistant strain( dTrs.) was resulted from moderately to high level larvae of Trs, and was subjected to selection pressure with chlorpyrifos as a selected agent for four successive generations, at the LC50 level. The obtained results revealed that LC50 value of Tss. and dTss. were 0.905 and 0.607, respectively. Also dTrs. strain was exhibited 14.47 fold of resistance as compared to 8.45 fold for Trs. Strain. Cross resistance spectrum was tested on dTss, Tss,, uTss .parent, Trs, and dTrs. Using 3 OP's compounds, one carbamate, 2 synthetic pyrethroids , and one demeloid. All strains tested proved that increase in resistance level was accompanied by steady increase in cross resistance of all tested compounds. All strains tested were in between vigor tolerance to true resistance ( positive cross resistance).Analysis of data showed that developed resistance strain exhibited a moderate level of resistance to the selected agent (chlorpyrifos). Degree of dominance of resistance in F1a ( dTss males × dTrs females) and F2b (dTrs males × dTss females) exhibited a moderate level of recessive. Reciprocal crosses in F1 progney indicated that increase in LC50 and slope in F2b ( heterozygous) was done. Enzyme activity of acid phosphatase (Acph) and alkakine phosphatase ( Alkph) and transaminases enzyme were established for all strains. Generally, it was obvious that chlorpyrifos resistance in S.littoralis expressed as a high activity of enzyme was more exhibited in the developed and hybrid strains, while traditional strains recorded a fluctuated slightly levels. | ||||
| Keywords | ||||
| Spodoptera littoralis; resistance; Susceptible; Enzyme | ||||
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