Growth of Lactococcus lactis subsp lactis in Milk Under Control of Culture Acidity
Kamel, D., Gomma, N., Osman, D., Hassan, A. (2017). Growth of Lactococcus lactis subsp lactis in Milk Under Control of Culture Acidity. EKB Journal Management System, 48(6), 32-39. doi: 10.21608/ajas.2017.7568
Dalia G. Kamel; Nanis H. Gomma; Dina M. Osman; A. I. Hassan. "Growth of Lactococcus lactis subsp lactis in Milk Under Control of Culture Acidity". EKB Journal Management System, 48, 6, 2017, 32-39. doi: 10.21608/ajas.2017.7568
Kamel, D., Gomma, N., Osman, D., Hassan, A. (2017). 'Growth of Lactococcus lactis subsp lactis in Milk Under Control of Culture Acidity', EKB Journal Management System, 48(6), pp. 32-39. doi: 10.21608/ajas.2017.7568
Kamel, D., Gomma, N., Osman, D., Hassan, A. Growth of Lactococcus lactis subsp lactis in Milk Under Control of Culture Acidity. EKB Journal Management System, 2017; 48(6): 32-39. doi: 10.21608/ajas.2017.7568

Growth of Lactococcus lactis subsp lactis in Milk Under Control of Culture Acidity

Assiut Journal of Agricultural Sciences
Article 3, Volume 48, Issue 6, December 2017, Page 32-39  XML PDF (403.81 K)
Document Type: Original Article
DOI: 10.21608/ajas.2017.7568
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Authors
Dalia G. Kamel* ; Nanis H. Gomma; Dina M. Osman; A. I. Hassan
Dairy Science Department, Faculty of Agriculture, Assiut University.
Abstract
The effect of control of culture acidity on growth kinetics of one homofermentative strains of lactic acid bacteria, namely., Lactococcus lactis subsp lactis  was evaluated. The bacterial growth was estimated on the base of colony forming units, developed titratable acidity and incubation time .Sterilized skim milk was used as a medium with incubation at 34°C.
Sampling had been carried out at zero time and each 2 hours intervals. After each sampling, a calculated volume of a liquid of 0.1 N sodium hydroxide was added to the culture to adjust the acidity of the remaining volume of the cultures to its initial value at zero time.
The obtained results indicated that:
1-    When Lactococcus lactis subsp lactis grown under control of culture acidity lag phase was not observed.
2-    Significant effect of acidity control was noticed on the growth rate. Exponential phase of growth was between 2ed and 12th hour of incubation. However, the same culture being grown with control of culture acidity the exponential phase of growth was at interval of time from zero up to 24th hour of incubation.
3-      The maximum cell population in case of the control sample was 124 x 105 CFU /ml after 12 hours of incubation. The corresponding values when growth was under control of culture acidity was by a maximum cell population of 35x106 CFU/ml after 32 hour of incubation.
Keywords
lactic acid bacteria; titratable acidity; colony count; culture acidity control; Mrs Media
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