Podocyte-associated messenger ribonucleic acids profile in urine of patients with idiopathic nephrotic syndrome and lupus nephritis.
HASSAN, A., Elokely, A., Waked, E., Shemis, M., Soliman, S. (2022). Podocyte-associated messenger ribonucleic acids profile in urine of patients with idiopathic nephrotic syndrome and lupus nephritis.. EKB Journal Management System, 28(6.2), 246-252. doi: 10.21608/zumj.2020.22866.1706
ANAS HASSAN HASSAN; Amir Mohammed Elokely; EMAM Abd Ellatif Waked; Mohamed Abbas Shemis; Sameh Abdel azeem Soliman. "Podocyte-associated messenger ribonucleic acids profile in urine of patients with idiopathic nephrotic syndrome and lupus nephritis.". EKB Journal Management System, 28, 6.2, 2022, 246-252. doi: 10.21608/zumj.2020.22866.1706
HASSAN, A., Elokely, A., Waked, E., Shemis, M., Soliman, S. (2022). 'Podocyte-associated messenger ribonucleic acids profile in urine of patients with idiopathic nephrotic syndrome and lupus nephritis.', EKB Journal Management System, 28(6.2), pp. 246-252. doi: 10.21608/zumj.2020.22866.1706
HASSAN, A., Elokely, A., Waked, E., Shemis, M., Soliman, S. Podocyte-associated messenger ribonucleic acids profile in urine of patients with idiopathic nephrotic syndrome and lupus nephritis.. EKB Journal Management System, 2022; 28(6.2): 246-252. doi: 10.21608/zumj.2020.22866.1706

Podocyte-associated messenger ribonucleic acids profile in urine of patients with idiopathic nephrotic syndrome and lupus nephritis.

Zagazig University Medical Journal
Article 37, Volume 28, Issue 6.2, November 2022, Page 246-252  XML PDF (374.37 K)
Document Type: Original Article
DOI: 10.21608/zumj.2020.22866.1706
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Authors
ANAS HASSAN HASSAN email orcid 1; Amir Mohammed Elokely2; EMAM Abd Ellatif Waked3; Mohamed Abbas Shemis4; Sameh Abdel azeem Soliman5
1Assistant Researcher at nephrology department, Theodor Bilharz Research Institute
2internal medicine department,faculty of medicine,zagazig university,zagazig,egypt
3Professor of Internal Medicine and Nephrology Theodor Bilharz Research Institute
4Professor of Biochemistry and Molecular Biology Theodor Bilharz Research Institute.
5Assistant professor of Internal Medicine and Nephrology Faculty of Medicine - Zagazig University
Abstract
Background: Podocytes associated proteins are essential in the maintenance of a healthy glomerular filtration barrier, a spectrum of different glomerular diseases occurs due to podocyte abnormalities. Urinary podocytes mRNAs are a more accurate tool for monitoring the progression of different glomerular diseases than proteinuria.
Methods: Quantification of podocyte mRNAs in urinary sediment by real time polymerase chain reaction (PCR) of idiopathic nephrotic syndrome, lupus nephritis patients (LN) and the healthy controls, each group contains 15 individuals to be correlated with proteinuria level and eGFR (by MDRD equation).
Results: the urinary podocyte mRNAs in both idiopathic nephrotic syndrome and LN groups differed significantly in comparison to that of controls, there was a significant correlation when comparing renal function tests and estimated glomerular filtration rate (by MDRD equation) between nephrotic, LN groups and control group, the value of podocin showed significant correlation in active LN subgroup with its corresponding value in the non-active LN subgroup.
Conclusions: This study has revealed that urine pellet podocyte mRNAs can be used as a tool for monitoring the progression of idiopathic nephrotic syndrome and lupus nephritis patients and that urinary podocin can be used as a marker for lupus nephritis activity.
Keywords
Podocytes; nephrotic syndrome; Lupus nephritis; Proteinuria
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