HIGH-RESOLUTION MELTING CURVE (HRM) ANALYSIS IN GENOTYPIC DISCRIMINATION OF CRYPTOSPORIDIUM ISOLATES FROM STOOL OF EGYPTIAN CHILDREN
GHALLAB, M., KHATER, M., TYLER, K., BOUZID, M., ABD ELMEGIED, A., EL-BADRY, A. (2018). HIGH-RESOLUTION MELTING CURVE (HRM) ANALYSIS IN GENOTYPIC DISCRIMINATION OF CRYPTOSPORIDIUM ISOLATES FROM STOOL OF EGYPTIAN CHILDREN. EKB Journal Management System, 48(3), 557-562. doi: 10.21608/jesp.2018.76553
MARWA M. I. GHALLAB; MONA M. KHATER; KEVIN M. TYLER; MAHA BOUZID; AZZA KAMAL ABD ELMEGIED; AYMAN A. EL-BADRY. "HIGH-RESOLUTION MELTING CURVE (HRM) ANALYSIS IN GENOTYPIC DISCRIMINATION OF CRYPTOSPORIDIUM ISOLATES FROM STOOL OF EGYPTIAN CHILDREN". EKB Journal Management System, 48, 3, 2018, 557-562. doi: 10.21608/jesp.2018.76553
GHALLAB, M., KHATER, M., TYLER, K., BOUZID, M., ABD ELMEGIED, A., EL-BADRY, A. (2018). 'HIGH-RESOLUTION MELTING CURVE (HRM) ANALYSIS IN GENOTYPIC DISCRIMINATION OF CRYPTOSPORIDIUM ISOLATES FROM STOOL OF EGYPTIAN CHILDREN', EKB Journal Management System, 48(3), pp. 557-562. doi: 10.21608/jesp.2018.76553
GHALLAB, M., KHATER, M., TYLER, K., BOUZID, M., ABD ELMEGIED, A., EL-BADRY, A. HIGH-RESOLUTION MELTING CURVE (HRM) ANALYSIS IN GENOTYPIC DISCRIMINATION OF CRYPTOSPORIDIUM ISOLATES FROM STOOL OF EGYPTIAN CHILDREN. EKB Journal Management System, 2018; 48(3): 557-562. doi: 10.21608/jesp.2018.76553

HIGH-RESOLUTION MELTING CURVE (HRM) ANALYSIS IN GENOTYPIC DISCRIMINATION OF CRYPTOSPORIDIUM ISOLATES FROM STOOL OF EGYPTIAN CHILDREN

Journal of the Egyptian Society of Parasitology
Article 9, Volume 48, Issue 3, December 2018, Page 557-562  XML PDF (522.11 K)
Document Type: Original Article
DOI: 10.21608/jesp.2018.76553
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Authors
MARWA M. I. GHALLAB1, 2; MONA M. KHATER3; KEVIN M. TYLER4; MAHA BOUZID4; AZZA KAMAL ABD ELMEGIED5; AYMAN A. EL-BADRY6
1Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University.+
2Department of Medical Parasitology, Faculty of Medicine, Kafr El- Sheikh University, Egypt.
3Department of Medical Parasitology, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt.
4Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, UK.
5Department of Pediatrics, Kasr Al-Ainy Faculty of Medicine, Cairo University, Egypt.
6Department of Medical Parasitology, Department of Microbiology-Medical Parasitology Section, College of Medicine, Imam Abdulrahman Bin Fasial University, Dammam, Saudi Arabia.
Abstract
Cryptosporidiosis is a recognized child infectious killer and the second cause of diarrheal disease and death in infants. Assessing Cryptosporidium spp. genetic diversity is a real goal to elucidate its transmission dynamics and to design preventive measures in absence of effective treatment. Cryptosporidium isolates in stool of Egyptian children were detected using Acid Fast (AF) staining, copro-nPCR/RFLP assay and real time PCR high-resolution melting (HRM) curve analysis assay. Stool samples were collected from 335 children complaining of diarrhea and other GIT symptoms, attending the outpatient clinic of Abu El Reesh hospital, Kasr Al-Ainy School of Medicine, Cairo University. Two genotypes C. hominis and C. parvum were identified in 43 isolates from Egyptian children by copro-nPCR targeting COWP gene and HRM assay. Real time PCR HRM curve analysis, a closed-tube genotyping method, targeting ITS-2 gene confirmed the results of copro-nPCR/RFLP. It is simple, rapid, has more sample throughput, analysis capacities and data storage with less carry-over contamination and cost.
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