Isolation and Molecular Characterization of Infectious Bronchitis Virus from Broiler Chickens, Egypt during 2014 -2016 | ||||
| Zagazig Veterinary Journal | ||||
| Article 2, Volume 45, Issue 1, March 2017, Page 11-18 PDF (298.32 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/zvjz.2017.7682 | ||||
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| Authors | ||||
Mohammed A. Lebdah1; Ahmed M. Hegazy1; Mohammed H. Hassan2; Mai Mohammed  2
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| 1Avian and Rabbit Medicine Department, Faculty of Veterniary Medicine University, Zagazig, 44511, Egypt | ||||
| 2Poultry Diseases Department, Animal Health Research Institute, P.O. Box 264-Dokki, Giza, 12618, Egypt | ||||
| Abstract | ||||
| In this study,surveillance on infectious bronchitis virus strains in 75 Egyptian broiler chicken flocks was performed using real-time RT-PCR at eight Governorates during 2014-2016. The birds in the selected flocks were suffering from respiratory and renal manifestations. The results of the present study showed that 31 (41%) samples were positive for IBV. All isolates were classified as variants using sequencing analysis of the obtained data and can be divided into 2 groups. Group I (variant) Egyptian IBV included isolates, EG-F728-1-2015-SP1, EG-F728-7-2015-SP1, EG-F862-3-2015-SP1, EG-F862-4-2015-SP1, EG-F888-2015-SP1, EG-F777-2015-SP1 and EG-F183-1-2016-SP1 which were very close to IBV-IS-1494-06 and had amino acid sequence identities between 87.5% to 96.2%. Group II (variant) Egyptian IBV included isolates, EG-F183-2-2016-SP1 and EG-F183-3-2016-SP1. These two IBV isolates were very close to IBV-IS/885 (variant 2) and they shared amino acid sequence identities from 92.3% to 97.1% with each other. In conclusion, different variants of IBV are present in broiler farms in high prevalence rate even in vaccinated flocks. | ||||
| Keywords | ||||
| IBV; broiler; Egypt; Sequencing; Variants | ||||
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