Detection of Shiga Toxin – Producing Escherichia coli in Raw and Pasteurized Milk
Ahmed, W., Samer, A. (2017). Detection of Shiga Toxin – Producing Escherichia coli in Raw and Pasteurized Milk. EKB Journal Management System, 45(1), 47-54. doi: 10.21608/zvjz.2017.7686
Waffa F. Ahmed; Amera Samer. "Detection of Shiga Toxin – Producing Escherichia coli in Raw and Pasteurized Milk". EKB Journal Management System, 45, 1, 2017, 47-54. doi: 10.21608/zvjz.2017.7686
Ahmed, W., Samer, A. (2017). 'Detection of Shiga Toxin – Producing Escherichia coli in Raw and Pasteurized Milk', EKB Journal Management System, 45(1), pp. 47-54. doi: 10.21608/zvjz.2017.7686
Ahmed, W., Samer, A. Detection of Shiga Toxin – Producing Escherichia coli in Raw and Pasteurized Milk. EKB Journal Management System, 2017; 45(1): 47-54. doi: 10.21608/zvjz.2017.7686

Detection of Shiga Toxin – Producing Escherichia coli in Raw and Pasteurized Milk

Zagazig Veterinary Journal
Article 6, Volume 45, Issue 1, March 2017, Page 47-54  XML PDF (140.44 K)
Document Type: Original Article
DOI: 10.21608/zvjz.2017.7686
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Authors
Waffa F. Ahmed1; Amera Samer email 2
1Food Hygiene Department, Animal Health Research Institute, Zagazig Provincial Lab, Egypt
2Microbiology Department, Animal Health Research Institute, Zagazig Provincial Lab, Egypt
Abstract
One hundred random samples of raw buffalo and pasteurized cow milk (50, each) were collected from different localities of Sharkia Governorate for the detection of E. coli. The isolates were screened by PCR for virulence associated genes as well as antibiotic sensitivity test to determine the most effective antimicrobial agent. Furthermore, an experimental study was carried out to detect the influence of pasteurization and processing of kariesh cheese and yoghurt on E. coli O111:H8 inoculated in milk with the concentration of 108 CFU/mL. The obtained results showed that the occurrence of E. coli in the examined raw buffalo and pasteurized cow milk samples were 66% and 30%, respectively. Serogrouping of E. coli isolates revealed that O128, O26 and O111 were recorded as the most frequent O-serogroups.  The sensitivity test showed that E. coli isolates were more sensitive to gentamicin (79.2%), followed by ciprofloxacin (70.8%) and colistin (68.8%). However, the examined isolates were completely resistant to erythromycin (100%) followed by sulphamethazole- trimethoprim (79.2%). Molecular identification of virulence associated genes revealed stx1, stx2 and eaeA genes. The experimental study showed that milk pasteurization was more effective on E. coli O111:H8 survival at refrigeration temperature compared with processed products such as kariesh cheese and yoghurt.
Keywords
E. coli; Serotypes; stx1; stx2; eaeA
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