MOLECULAR EPIDEMIOLOGICAL STUDIES ON TRYPANOSOMA EVANSI TYPE A AND TYPE B IN CAMELS (CAMELUS DROMEDARIES) FROM FIVE DIFFERENT REGIONS OF SAUDI ARABIA USING THE ITS1 RDNA AND ROTAT 1.2 VSG GENE
ALANAZI, A., PUSCHENDORF, R., ALYOUSIF, M., AL-KHALIFA, M., ALHARBI, S., AL SHEHRI, Z., SAID, A., ALANAZI, I., AL-MOHAMMED, H. (2018). MOLECULAR EPIDEMIOLOGICAL STUDIES ON TRYPANOSOMA EVANSI TYPE A AND TYPE B IN CAMELS (CAMELUS DROMEDARIES) FROM FIVE DIFFERENT REGIONS OF SAUDI ARABIA USING THE ITS1 RDNA AND ROTAT 1.2 VSG GENE. EKB Journal Management System, 48(2), 241-250. doi: 10.12816/0050431
ABDULLAH D. ALANAZI; R. PUSCHENDORF; MOHAMED S. ALYOUSIF; MOHAMED S. AL-KHALIFA; SAMIR A. ALHARBI; ZAFER S. AL SHEHRI; ASHRAF E. SAID; IBRAHIM O. ALANAZI; HAMDAN I. AL-MOHAMMED. "MOLECULAR EPIDEMIOLOGICAL STUDIES ON TRYPANOSOMA EVANSI TYPE A AND TYPE B IN CAMELS (CAMELUS DROMEDARIES) FROM FIVE DIFFERENT REGIONS OF SAUDI ARABIA USING THE ITS1 RDNA AND ROTAT 1.2 VSG GENE". EKB Journal Management System, 48, 2, 2018, 241-250. doi: 10.12816/0050431
ALANAZI, A., PUSCHENDORF, R., ALYOUSIF, M., AL-KHALIFA, M., ALHARBI, S., AL SHEHRI, Z., SAID, A., ALANAZI, I., AL-MOHAMMED, H. (2018). 'MOLECULAR EPIDEMIOLOGICAL STUDIES ON TRYPANOSOMA EVANSI TYPE A AND TYPE B IN CAMELS (CAMELUS DROMEDARIES) FROM FIVE DIFFERENT REGIONS OF SAUDI ARABIA USING THE ITS1 RDNA AND ROTAT 1.2 VSG GENE', EKB Journal Management System, 48(2), pp. 241-250. doi: 10.12816/0050431
ALANAZI, A., PUSCHENDORF, R., ALYOUSIF, M., AL-KHALIFA, M., ALHARBI, S., AL SHEHRI, Z., SAID, A., ALANAZI, I., AL-MOHAMMED, H. MOLECULAR EPIDEMIOLOGICAL STUDIES ON TRYPANOSOMA EVANSI TYPE A AND TYPE B IN CAMELS (CAMELUS DROMEDARIES) FROM FIVE DIFFERENT REGIONS OF SAUDI ARABIA USING THE ITS1 RDNA AND ROTAT 1.2 VSG GENE. EKB Journal Management System, 2018; 48(2): 241-250. doi: 10.12816/0050431

MOLECULAR EPIDEMIOLOGICAL STUDIES ON TRYPANOSOMA EVANSI TYPE A AND TYPE B IN CAMELS (CAMELUS DROMEDARIES) FROM FIVE DIFFERENT REGIONS OF SAUDI ARABIA USING THE ITS1 RDNA AND ROTAT 1.2 VSG GENE

Journal of the Egyptian Society of Parasitology
Article 1, Volume 48, Issue 2, August 2018, Page 241-250  XML PDF (834.87 K)
Document Type: Original Article
DOI: 10.12816/0050431
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Authors
ABDULLAH D. ALANAZI1; R. PUSCHENDORF2; MOHAMED S. ALYOUSIF3; MOHAMED S. AL-KHALIFA3; SAMIR A. ALHARBI1; ZAFER S. AL SHEHRI1; ASHRAF E. SAID4, 5; IBRAHIM O. ALANAZI6; HAMDAN I. AL-MOHAMMED7
1Department of Biological Science, Faculty of Science and Humanities, Shaqra University, P.O. Box 1040, Ad-Dawadimi 11911.
2School of Biological Sciences, Plymouth University, Drake Circus, Plymouth, PL4 8AA, UK.
3Department of Zoology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451.
4Department of Biological Science, Faculty of Science and Humanities, Shaqra University, P.O. Box 1040, Ad-Dawadimi 11911.+
5Department of Zoology, Faculty of Science, Damietta University, New Damietta, Damietta, Egypt.
6The National Centre for Genomic Technology, King Abdulaziz City for Science and technology, P.O. Box 6086, Riyadh 11442.
7Department of Parasitology, Faculty of Medicine, King Faisal University P.O. Box 400, AlAhsa 31982, Saudi Arabia.
Abstract
Trypanosoma evansi is the most widespread of the pathogenic salivarian trypanosomes and cause a serious disease called (surra) that is affect the domestic animals such camels and horses in Tropical and subtropical countries and often leads to reduced productivity and economic losses. Therefore, the objectives of the present study were to determine the prevalence rates of trypanosomiasis using polymerase chain reaction (PCR) among camels from five different regions of Saudi Arabia and to sequence and characterized the T. evansi from these animals. In the current study, 832 camel blood samples collected from five different regions of Saudi Arabia for detecting T. evansi. A generic ITS1-PCR and RoTat 1.2 VSG gene were applied in this study to analyze camels’ blood samples. Molecular analysis was performed using ITS1-PCR
which showed that the highest prevalence of trypanosomes was observed in Al-Qaseem province (50.1%) followed Riyadh province (49%), whereas in Hail and the Northern Borders, there were fewer infections with trypanosomes (28.4% &17.6%), respectively. PCR amplification was carried out targeting RoTat 1.2 VSG gene on TS1-positive samples and some of them were negative for RoTat1.2. The test negative in RoTat 1.2 PCR but ITS1 PCR positive could suggest T. evansi type B. Presence of T. evansi type B is interest to the international community, as this has a message to redesign the existing molecular and serological diagnostic markers. However, to our knowledge this the first study demonstrating T. evansi type B out of Africa.
Keywords
Trypanosoma evansi; camels; ITS1 rDNA; RoTat 1.2; PCR; Saudi Arabia
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