AN ASSESSMENT OF THE ANTIMICROBIAL ACTIVITY OF SARCOPHAGA ARGYROSTOMA HEMOLYMPH AGAINST PATHOGENIC MICROORGANISMS
MOSELHY, W., ABDEL-AZIZ, M. (2018). AN ASSESSMENT OF THE ANTIMICROBIAL ACTIVITY OF SARCOPHAGA ARGYROSTOMA HEMOLYMPH AGAINST PATHOGENIC MICROORGANISMS. EKB Journal Management System, 48(2), 327-334. doi: 10.12816/0050439
WALAA A. MOSELHY; MARWA M. ABDEL-AZIZ. "AN ASSESSMENT OF THE ANTIMICROBIAL ACTIVITY OF SARCOPHAGA ARGYROSTOMA HEMOLYMPH AGAINST PATHOGENIC MICROORGANISMS". EKB Journal Management System, 48, 2, 2018, 327-334. doi: 10.12816/0050439
MOSELHY, W., ABDEL-AZIZ, M. (2018). 'AN ASSESSMENT OF THE ANTIMICROBIAL ACTIVITY OF SARCOPHAGA ARGYROSTOMA HEMOLYMPH AGAINST PATHOGENIC MICROORGANISMS', EKB Journal Management System, 48(2), pp. 327-334. doi: 10.12816/0050439
MOSELHY, W., ABDEL-AZIZ, M. AN ASSESSMENT OF THE ANTIMICROBIAL ACTIVITY OF SARCOPHAGA ARGYROSTOMA HEMOLYMPH AGAINST PATHOGENIC MICROORGANISMS. EKB Journal Management System, 2018; 48(2): 327-334. doi: 10.12816/0050439

AN ASSESSMENT OF THE ANTIMICROBIAL ACTIVITY OF SARCOPHAGA ARGYROSTOMA HEMOLYMPH AGAINST PATHOGENIC MICROORGANISMS

Journal of the Egyptian Society of Parasitology
Article 9, Volume 48, Issue 2, August 2018, Page 327-334  XML PDF (586 K)
Document Type: Original Article
DOI: 10.12816/0050439
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Authors
WALAA A. MOSELHY1; MARWA M. ABDEL-AZIZ2
1Department of Zoology, Faculty of Science, Al-Azhar University (Girls), Egypt.
2Regional Center of Mycology and Biotechnology, Al-Azhar University, Cairo, Egypt.
Abstract
The pathogenic microorganisms are exceptionally adept to acquiring resistance to antibiotic and antiseptic drugs, so new strategies are there for needed to deal with this threat. Antimicrobial peptides from a wide spectrum of insects possess potent microbicidal properties against disease-related microorganisms. This study investigated the antimicrobial activity of maggot hemolymph from Sarcophaga argyrostama, a common species of flesh flies in Egypt. The crude hemolymph of the third instar larvae was tested against selected pathogenic strains of Gram positive bacteria, Streptococcus pyogenes, Staphylococcus aureus, S. epidermidis, Bacillus subtilis and Micrococcus lutea; Gram negative bacteria, Pseudomonas aeruginosa, Proteous vulgaris, Salmonella typhimurium, Klebsiella pneumo-niae and Escherichia coli and Fungi, Aspergillus niger, Syncephalastrum racemosum, Geotrichum candidum, Candida albicans, Microsporum canis and Trichophyton mentagro-phytes. The results showed that hemolymph was more effective against Gram positive bacteria especially Bacillus subtilis and effective against the filamentous fungus Geotrichum candidum. The most affected bacterial
and fungal species were examined by TEM to study the antimicrobial peptides action in the hemolymph. Also, crude hemolymph was subjected to SDS-PAGE to estimate the proteins number and molecular weight.
Keywords
Sarcophaga Argyrostoma; Hemolymph; antimicrobial activity; microorganisms
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