The role of Platelet Rich Fibrin in bone defect regeneration | ||||
Egyptian Dental Journal | ||||
Article 3, Volume 64, Issue 2 - April (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), April 2018, Page 1161-1170 PDF (2.31 MB) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/edj.2018.77001 | ||||
View on SCiNiTO | ||||
Authors | ||||
Mazen T Abou ElKhier1; Laila E Amin2; Nagla M Salama3 | ||||
1Associate Professor of Oral Biology Department, Faculty of Dentistry, Mansoura University | ||||
2Lecturer of Oral Biology Department, Faculty of Dentistry, Mansoura University | ||||
3Associate professor of Oral Pathology Department, Faculty of Dentistry, Mansoura University | ||||
Abstract | ||||
Background: Natural tissue regeneration relies on a cocktail of signaling molecules and growth factors. During natural wound healing, activated platelets concentrate in the wound area and secrete factors that play a role in wound healing. Platelet rich fibrin (PRF) represents a revolutionary step in the platelet gel therapeutic concept. It needs only centrifugation of the natural blood without additives. Objectives: This present study designed to evaluate the role of PRF on regeneration of bone defect. Design: In this study 24New Zealand white male rabbits were used with bilateral cortical bony defects in the submental mandibular area, divided into two groups. The right bone defects were filled with PRF (experimental) and the left bone defects kept empty (control). Each group was equally subdivided into three subgroups; rabbits were sacrificed at 2nd, 4th and 6th weeks. Immunohistochemical analysis for collagen I and vascular endothelial growth factor (VEGF) were done. Results: The histological findings illustrated that all experimental subgroups showed more deposition of osteoid tissue and well organized bone trabeculae. Immunohistochemical findings of collagen I revealed statistically to be the highest in PRF group. While VEGF illustrated a positive expression by bone marrow stromal cells and bone tissue cells in different value and the PRF group recorded the highest value. Conclusion: It was concluded that PRF can accelerate bone regeneration and had effect on collagen I and VEGF expression. | ||||
Keywords | ||||
Bone defect; Platelet rich fibrin; Collagen I; VEGF | ||||
Statistics Article View: 120 PDF Download: 123 |
||||