Phylogenetic relationships and taxonomy of three species of family Lethrinidae in the Red Sea, Egypt
B. E., A., Ahmed, M. I., A., Madkour F. F., M., Hanafy M. H., H. (2018). Phylogenetic relationships and taxonomy of three species of family Lethrinidae in the Red Sea, Egypt. EKB Journal Management System, 22(1), 17-24. doi: 10.21608/ejabf.2018.7710
Abdelmeneam B. E. B. E.; Ahmed, M. I. Ahmed, M. I.; Madkour F. F. Madkour F. F.; Hanafy M. H. Hanafy M. H.. "Phylogenetic relationships and taxonomy of three species of family Lethrinidae in the Red Sea, Egypt". EKB Journal Management System, 22, 1, 2018, 17-24. doi: 10.21608/ejabf.2018.7710
B. E., A., Ahmed, M. I., A., Madkour F. F., M., Hanafy M. H., H. (2018). 'Phylogenetic relationships and taxonomy of three species of family Lethrinidae in the Red Sea, Egypt', EKB Journal Management System, 22(1), pp. 17-24. doi: 10.21608/ejabf.2018.7710
B. E., A., Ahmed, M. I., A., Madkour F. F., M., Hanafy M. H., H. Phylogenetic relationships and taxonomy of three species of family Lethrinidae in the Red Sea, Egypt. EKB Journal Management System, 2018; 22(1): 17-24. doi: 10.21608/ejabf.2018.7710

Phylogenetic relationships and taxonomy of three species of family Lethrinidae in the Red Sea, Egypt

Egyptian Journal of Aquatic Biology and Fisheries
Article 2, Volume 22, Issue 1, January 2018, Page 17-24  XML PDF (522.14 K)
Document Type: Original Article
DOI: 10.21608/ejabf.2018.7710
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Authors
Abdelmeneam B. E. B. E.1; Ahmed, M. I. Ahmed, M. I.2; Madkour F. F. Madkour F. F.2; Hanafy M. H. Hanafy M. H.2
1Marine Science Department, Faculty of Science, Port Said University, Port Said, Egypt
2Marine Science Department, Faculty of Science, Suez Canal University, Ismailia, Egypt
Abstract
Species of family Lethrinidae are of a great commercial importance in Egypt. Recent researches proved that we are far from having a full list of the family species diversity in Egypt. The purpose of this study was to clarify the phylogeny of commercial fish species of family Lethrinidae in the Egyptian Red Sea. Three commercial species of Lethrinidae (Lethrinus mahsena, Lethrinus nebulosus and Monotaxis grandoculis) were obtained from northern Red Sea proper. Samples were identified based on morphological characters. Identification was confirmed using DNA barcoding technique. DNA extraction was performed using Phenol-chloroform extraction method. In PCR, About 655 bp were amplified from the 5' region of the COI gene from mitochondrial DNA, then successful amplifications were sequenced. By Combining our sequence results with sequences of some species of Family Lethrinidae submitted to the GenBank, phylogenetic trees were constructed using p-distance Neighbor-Joining method. Results indicated that the use of DNA barcoding technique for emperor fish and big eye bream identification was successful, not only between different species of the family, but also within species, indicating the possible presence of cryptic species complex and the possible discovery of new genetic diversity. Of the three examined species from the Egyptian coasts; Monotaxis grandoculis showed the lowest genetic differences. Lethrinus mahsena showed the highest variations. 
Keywords
Lethrinidae; Phylogeny; DNA barcoding; Taxonomy; Red Sea
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