Evaluation of Antibacterial Effect of Diode laser 980 nm, Triple Antibiotic Past, and Calcium Hydroxide on Enterococcus Faecalis Biofilm | ||||
Egyptian Dental Journal | ||||
Article 48, Volume 64, Issue 3 - July (Fixed Prosthodontics, Dental Materials, Conservative Dentistry & Endodontics), July 2018, Page 2863-2870 PDF (697.32 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/edj.2018.77359 | ||||
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Authors | ||||
Maram Farouk Obeid1; Mona Saad Nour2 | ||||
1Lecturer of Endodontics, Department of Endodontics, Faculty of Dentistry, Ain Shams University. | ||||
2Lecturer of Medical Microbiology and Immunology, Department of Medical Microbiology and Immunology Faculty of Medicine- Ain Shams University | ||||
Abstract | ||||
Aim: The purpose of this study is to evaluate the anti-biofilm capacity of Diode laser 980 nm, triple antibiotic mixture and calcium hydroxide. Methods: Eighty five single-rooted teeth with mature apices were prepared using Protaper Universal rotary nickel titanium system till size # F4 then contaminated with E.faecalis. Irrigation done using 2.5% sodium hypochlorite followed by 17% EDTA. Samples were divided into 4 groups (n= 20) according to the irrigant activation method. Laser group,TAP group, Ca(OH)2group and the control group. Residual bacteria were plated onto Brain Heart Infusion media and determined as colony-forming units (CFU mL-1). Data were analyzed using one way ANOVA followed by performance of Tukey post hoc tests. Significance was set at p < 0.05. Results: There was a statistically significant reduction in the mean numbers of colony-forming units among all groups. However, none of the activation methods was able to kill E. faecalis biofilm completely. The Laser group behaved most effectively among all groups. Conclusion: The adjunctive use of 980nm laser is an effective method for bacterial reduction after chemo-mechanical instrumentation of the root canal. | ||||
Keywords | ||||
Diode laser; TAP; antibiofilm activity | ||||
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