Progranulin Protects Rat Testis From Ischaemic Reperfusion Injury | ||||
| The Medical Journal of Cairo University | ||||
| Article 33, Volume 87, December, December 2019, Page 4269-4276 PDF (652.29 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/mjcu.2019.77430 | ||||
 View on SCiNiTO | ||||
| Author | ||||
| EMAN R. ABOZAID, M.D.; NAGLAA A. AHMED, M.D. | ||||
| The Department of Physiology*, Faculty of Medicine, Zagazig University, Egypt and Physiology Department**, Faculty of Medicine, Najran University, KSA | ||||
| Abstract | ||||
| Abstract Background: Ischemic reperfusion (I/R) damage of the testis is a common problem, it is mainly caused by testicular torsion and detorsion. Progranulin (PGRN) has been reported to have prominent anti-inflammatory and protective effects on I/R injury in the heart, brain and kidney, however, its effects on testicular I/R lesion have not been evaluated. Aim of Study: To examine the effects of progranulin on rat testicular ischaemia/reperfusion induced by torsion/ detor-sion and the possible underlying mechanisms. Material and Methods: In this study, rats were divided into 4 groups: (i) Sham; (ii) Testicular ischemia/reperfusion (I/R), 2h of ischemia followed by 6 h of reperfusion; (iii) I/R treated by PGRN (0.01mg/rat) administered 1.5h after the induction of ischaemia. (iv) I/R treated by PGRN (0.1mg/rat) administered 1.5h after development of ischemia. At the end of the study, some testicular oxidative stress markers, inflam-matory, apoptotic markers and serum testosterone were meas-ured, in addition to histopathologic study. Results: Significant decreases in serum testosterone levels, testicular superoxide dismutase (SOD) and glutathione per-oxidase (Gpx) activity with increase in tissue malondialdehyde (MDA) were observed in I/R group when compared with controls. These antioxidant levels were increased in PGRN treated groups with significant decrease in MDA levels. While an increase in tumor necrosis factor-alpha (TNF-a), IL-1b, caspase-8 and caspase-3 levels were found in the torsion/ detorsion group, significant decrease in the levels of these inflammatory cytokines and apoptotic markers were observed in PRGR treated groups in a dose dependent maner. Conclusion: These results demonstrate that PGRN signif-icantly produced protective effects on testicular tissue damage through antioxidant, anti-inflammatory and antiapoptotic mechanisms. | ||||
| Keywords | ||||
| Progranulin – Ischaemia – Reperfusion – Testis | ||||
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