THERAPEUTIC EFFICACY OF A CYSTEINE PROTEASES INHIBITOR (PHENYL VINYL SULFONE) EITHER ALONE OR COMBINED WITH NIGELLA SATIVA ON EXPERIMENTAL CRYPTOSPORIDIOSIS
ZAKI, W., EL-AMIR, Y. (2018). THERAPEUTIC EFFICACY OF A CYSTEINE PROTEASES INHIBITOR (PHENYL VINYL SULFONE) EITHER ALONE OR COMBINED WITH NIGELLA SATIVA ON EXPERIMENTAL CRYPTOSPORIDIOSIS. EKB Journal Management System, 48(1), 165-174. doi: 10.21608/jesp.2018.77484
WAFAA M. ZAKI; YASMIN O. EL-AMIR. "THERAPEUTIC EFFICACY OF A CYSTEINE PROTEASES INHIBITOR (PHENYL VINYL SULFONE) EITHER ALONE OR COMBINED WITH NIGELLA SATIVA ON EXPERIMENTAL CRYPTOSPORIDIOSIS". EKB Journal Management System, 48, 1, 2018, 165-174. doi: 10.21608/jesp.2018.77484
ZAKI, W., EL-AMIR, Y. (2018). 'THERAPEUTIC EFFICACY OF A CYSTEINE PROTEASES INHIBITOR (PHENYL VINYL SULFONE) EITHER ALONE OR COMBINED WITH NIGELLA SATIVA ON EXPERIMENTAL CRYPTOSPORIDIOSIS', EKB Journal Management System, 48(1), pp. 165-174. doi: 10.21608/jesp.2018.77484
ZAKI, W., EL-AMIR, Y. THERAPEUTIC EFFICACY OF A CYSTEINE PROTEASES INHIBITOR (PHENYL VINYL SULFONE) EITHER ALONE OR COMBINED WITH NIGELLA SATIVA ON EXPERIMENTAL CRYPTOSPORIDIOSIS. EKB Journal Management System, 2018; 48(1): 165-174. doi: 10.21608/jesp.2018.77484

THERAPEUTIC EFFICACY OF A CYSTEINE PROTEASES INHIBITOR (PHENYL VINYL SULFONE) EITHER ALONE OR COMBINED WITH NIGELLA SATIVA ON EXPERIMENTAL CRYPTOSPORIDIOSIS

Journal of the Egyptian Society of Parasitology
Article 19, Volume 48, Issue 1, April 2018, Page 165-174  XML PDF (647.88 K)
Document Type: Original Article
DOI: 10.21608/jesp.2018.77484
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Authors
WAFAA M. ZAKI1, 2; YASMIN O. EL-AMIR3, 4
1Department of Medical Parasitology, Faculty of Medicine, Suez Canal University, Egypt.+
2Department of Medical Laboratory Technology, Faculty of Applied Medical Science, Jazan University, KSA.
3Department of Medical Laboratory Technology, Faculty of Applied Medical Science, Jazan University, KSA.+
4Department of Pathology and Clinical Pathology, Faculty of Veterinary Medicine, Assiut University, Egypt.
Abstract
The present study evaluated the efficacy of a cysteine protease inhibitor (PVS) either alone or combined with Nigella sativa in treatment of cryptosporidiosis in immunosuppressed mice. Seventy two mice were divided equally into eight groups: a normal control group (G1). Other received dexamethasone to induce immunosuppression (G2) acted as a dexamethasone immunosuppressed control. Six immunosuppressed groups were inoculated with infective dose of Cryptosporidum oocycts: (G3) infected control, (G4 & G5) treated with PVS alone in a dose of 50mg/kg & 100mg/kg respectively, (G6 & G7) treated with same dose of PVS and combined with NS 500mg/kg, and (G8) was treated with Paromomycin 250mg/kg. Oocysts shedding were monitored from 2nd to 24th day post-infection. After mice scarification, blood was assessed for hepatorenal drug toxicity. Sections of ileum were subjected to histopathological examination. Mice treated with PVS 100mg/kg combined with NS showed marked improvement with highest significant efficacy (P ≤0.01) diminished oocyst shedding in G6 & G7 by 91% & 94% respectively, versus its efficacy when given alone in G4 & G5 by 83% & 87% respectively or when compared with paromomycin effect in G8 (81%). PVS in different doses showed no toxic effect on hepatorenal parameters.
Keywords
cryptosporidiosis; Cysteine proteases inhibitor; Nigella Sativa; Experimental study
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