The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus
Kamel, K., Khafagy, A., Bassiouni, A., Afify, M., Rabie, N. (2010). The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus. EKB Journal Management System, 20(1), 342-350. doi: 10.21608/jvmr.2020.77638
K. M. Kamel; A. Khafagy; A. A. Bassiouni; M. A. Afify; N. S. Rabie. "The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus". EKB Journal Management System, 20, 1, 2010, 342-350. doi: 10.21608/jvmr.2020.77638
Kamel, K., Khafagy, A., Bassiouni, A., Afify, M., Rabie, N. (2010). 'The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus', EKB Journal Management System, 20(1), pp. 342-350. doi: 10.21608/jvmr.2020.77638
Kamel, K., Khafagy, A., Bassiouni, A., Afify, M., Rabie, N. The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus. EKB Journal Management System, 2010; 20(1): 342-350. doi: 10.21608/jvmr.2020.77638

The prevalence of Infectious Bronchitis (IB) outbreaks in some chicken farms: II. Molecular characterization of field isolates of IB virus

Journal of Veterinary Medical Research
Article 51, Volume 20, Issue 1, March 2010, Page 342-350  XML PDF (353.42 K)
Document Type: Original Article
DOI: 10.21608/jvmr.2020.77638
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Authors
K. M. Kamel1; A. Khafagy2; A. A. Bassiouni3; M. A. Afify3; N. S. Rabie3
1Department of Poultry Diseases, National Research Center, Cairo
2Department of Poultry Diseases, Animal Health Research Institute, Dokki, Giza
3Department of Poultry Diseases, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
Abstract
Twenty five isolates of IBV were isolated from 36 broiler and layer chicken farms collected from 13 governorates during 2 years started from January 2003. All the examined farms were vaccinated using the commercial live IB-H120 vaccine in addition to the IB-inactivated vaccine in the layer farm. The viruses were isolated and identified previously by chicken embryo, CEK cell culture inoculation. Isolates subjected to RT-PCR. Four isolates; three broiler farms and one from layer farm were genotyped using S1 partial gene sequencing. Typing of the four isolates using S1 partial gene sequencing, revealed that the isolated IBV strains showed homology to Asia, Europe, USA and Middle East strains.
Keywords
prevalence; infectious; bronchitis; IB; Outbreaks; chicken; farms; Molecular; Characterization; field; isolates; virus
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