Comparison between molecular and classical techniques for identification of Mycoplasma species isolated from mastitic ruminants
Hassan, W., A. El-Shabrawy, M., Sadek, E. (2010). Comparison between molecular and classical techniques for identification of Mycoplasma species isolated from mastitic ruminants. EKB Journal Management System, 20(2), 7-15. doi: 10.21608/jvmr.2020.77645
W. H. Hassan; Mona A. El-Shabrawy; E. G. Sadek. "Comparison between molecular and classical techniques for identification of Mycoplasma species isolated from mastitic ruminants". EKB Journal Management System, 20, 2, 2010, 7-15. doi: 10.21608/jvmr.2020.77645
Hassan, W., A. El-Shabrawy, M., Sadek, E. (2010). 'Comparison between molecular and classical techniques for identification of Mycoplasma species isolated from mastitic ruminants', EKB Journal Management System, 20(2), pp. 7-15. doi: 10.21608/jvmr.2020.77645
Hassan, W., A. El-Shabrawy, M., Sadek, E. Comparison between molecular and classical techniques for identification of Mycoplasma species isolated from mastitic ruminants. EKB Journal Management System, 2010; 20(2): 7-15. doi: 10.21608/jvmr.2020.77645

Comparison between molecular and classical techniques for identification of Mycoplasma species isolated from mastitic ruminants

Journal of Veterinary Medical Research
Article 2, Volume 20, Issue 2, March 2010, Page 7-15  XML PDF (509.88 K)
Document Type: Original Article
DOI: 10.21608/jvmr.2020.77645
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Authors
W. H. Hassan1; Mona A. El-Shabrawy2; E. G. Sadek2
1Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, BeniSuef University, Beni-Suef, Egypt
2Department of Microbiology and Immunology, National Research Centre, Cairo, Egypt
Abstract
A total of 165 cows, 19 buffaloes, 192 sheep and 118 goats were examined for detection of Mycoplasma mastitis. The results revealed that 114 (69.59%) and 6 (31.57%) were clinically mastitic cows and buffaloes respectively while 51 (30.9%) and 13 (68.42%) were apparently healthy cows and buffaloes respectively. On examining the apparently healthy cows and buffaloes, 67 (32.84%) and 18 (34.61%) were subclinically mastitic cows and buffaloes respectively. Mycoplasmas were isolated in percentages of 8.9%, 5.5% from subclinically mastitic cows and buffaloes respectively and in percentages of 12.97%, 12.5% from clinically mastitic cows and buffaloes respectively. M. bovis was isolated from 8 (32%) and M. bovigenitalium from 7 (28%) and 10 (40%) unidentified Mycoplasma. Isolation of Mycoplasma from udder tissue in cows and buffaloes were in a percentage of 28.5% in cows while no Mycoplasma isolates were obtained from buffaloes' udder tissues. Application of PCR technique on these isolates and some of the negative samples was positive 100%. On the other hand, the results revealed that 82 of 192 (42.7%) and 43 of 118 (36.44) of the examined sheep and goats respectively were clinically mastitic. Isolation of Mycoplasma was from 11 (13.41%) and 17 (39.53%) of the examined sheep and goat respectively. Identification of these isolates revealed 8 (29%) M. agalactiae isolates and 20 (71%) unidentified Mycoplasma spp. Application of PCR technique on traditionally identified M. agalactiae isolates revealed negative results on using M. agalactiae specific primer while positive results were obtained for the same 8 isolates (100%) on using M. bovis specific primer.
Keywords
Comparison; Molecular; techniques; Mycoplasma; isolated; mastitic; ruminants
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