Molecular characterization of Corynebacterium pseudotuberculosis isolated from sheep by random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR)
Goda, A., Mahmoud, M., Osman, W., Ziada, N., Moussa, I. (2007). Molecular characterization of Corynebacterium pseudotuberculosis isolated from sheep by random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR). EKB Journal Management System, 17(1), 39-49. doi: 10.21608/jvmr.2007.77892
Azza S.A. Goda; Mona A. Mahmoud; Wafaa A. Osman; N.A. Ziada; I. M. Moussa. "Molecular characterization of Corynebacterium pseudotuberculosis isolated from sheep by random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR)". EKB Journal Management System, 17, 1, 2007, 39-49. doi: 10.21608/jvmr.2007.77892
Goda, A., Mahmoud, M., Osman, W., Ziada, N., Moussa, I. (2007). 'Molecular characterization of Corynebacterium pseudotuberculosis isolated from sheep by random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR)', EKB Journal Management System, 17(1), pp. 39-49. doi: 10.21608/jvmr.2007.77892
Goda, A., Mahmoud, M., Osman, W., Ziada, N., Moussa, I. Molecular characterization of Corynebacterium pseudotuberculosis isolated from sheep by random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR). EKB Journal Management System, 2007; 17(1): 39-49. doi: 10.21608/jvmr.2007.77892

Molecular characterization of Corynebacterium pseudotuberculosis isolated from sheep by random amplified polymorphic DNApolymerase chain reaction (RAPD-PCR)

Journal of Veterinary Medical Research
Article 8, Volume 17, Issue 1, March 2007, Page 39-49  XML PDF (3.39 MB)
Document Type: Original Article
DOI: 10.21608/jvmr.2007.77892
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Authors
Azza S.A. Goda1; Mona A. Mahmoud2; Wafaa A. Osman1; N.A. Ziada1; I. M. Moussa3
1Animal Health Department, Desert Research Center, Materia,Cairo, Egypt.
2Animal Health Department, Desert Research Center, Materia,Cairo, Egypt
3Department. of Microbiology, Faculty of Veterinary Medicine, Cairo University, Gizza, Egypt
Abstract
The phenogenetic and phylogenetic relationships among 7 identified strains of C.
pseudotuberculosis isolated from sheep were checked by PCR specific primers demonstrated with random amplified polymorphic DNA – polymerase chain reaction (RAPD – PCR) by using five arbitrarily primers. C pseudotuberculosis isolates were distinguished according to the banding patterns of their amplified DNA on agarose gel. The variation can be used for diagnostic differentiation among C.  pseudotuberculosis strains. Differences were observed in RAPD patterns between the 7 isolates and this may be due to the presence of novel serovars of this microorganism.
Keywords
Characterization; Molecular; Corynebacterium; pseudotuberculosis; isolated; sheep; random; amplified; polymorphic; DNA; Polymerase; RAPD-PCR
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