Protein analysis for comparison between Salmonellae isolated from different poultry species
El-Zeedy, S., Eldeen, H., Badr, J. (2007). Protein analysis for comparison between Salmonellae isolated from different poultry species. EKB Journal Management System, 17(2), 1-9. doi: 10.21608/jvmr.2007.77902
Seham A. El-Zeedy; Hussein K. Eldeen; Jihan M. Badr. "Protein analysis for comparison between Salmonellae isolated from different poultry species". EKB Journal Management System, 17, 2, 2007, 1-9. doi: 10.21608/jvmr.2007.77902
El-Zeedy, S., Eldeen, H., Badr, J. (2007). 'Protein analysis for comparison between Salmonellae isolated from different poultry species', EKB Journal Management System, 17(2), pp. 1-9. doi: 10.21608/jvmr.2007.77902
El-Zeedy, S., Eldeen, H., Badr, J. Protein analysis for comparison between Salmonellae isolated from different poultry species. EKB Journal Management System, 2007; 17(2): 1-9. doi: 10.21608/jvmr.2007.77902

Protein analysis for comparison between Salmonellae isolated from different poultry species

Journal of Veterinary Medical Research
Article 1, Volume 17, Issue 2, March 2007, Page 1-9  XML PDF (256.54 K)
Document Type: Original Article
DOI: 10.21608/jvmr.2007.77902
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Authors
Seham A. El-Zeedy1; Hussein K. Eldeen1; Jihan M. Badr2
1Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo Egypt.
2Animal Health Research Institute, Dokki Giza, Egypt.
Abstract
A total of 620 egg samples from different species (chickens, ducks and ostriches) and 1615 poultry samples (chickens, ducks, pigeons, quails, turkeys and ostriches) were examined for salmonella infection. 12 salmonella isolates were obtained from the egg samples (1.9%) and 67 isolates from poultry samples (4.1%). Salmonella isolates were serotyped into S. enteritidis (25 isolates), S. typhimurium (17 isolates), S. infantis (12 isolates), S. montivideo (7 isolates), 3 isolates for each of S. rubislaw and S. cerro , 2 isolates for each of S. virginia, S. agona, S. poona, and S. derby and 1 isolate for each of S. sandiago and S. kentucky. The incidence of isolation from different poultry species was discussed in details. Antibiogram of the isolated salmonellae against 10 different antibiotics revealed that norofloxacin, ciprofloxacin, cepheridin and gentamycin gave the highest activity against different salmonella isolates while amoxicillin, tetracycline, and nitrofurantoin showed the highest resistance rate. Pathogenicity of the isolated serovars was tested in chickens. All isolates were found pathogenic with various degree of virulence. SDSPAGE protein analysis for the salmonella isolated form different poultry species revealed 12 protein bands ranged from 22-289 kDa. The differences were insufficient for reliable differentiation between the isolates and accordingly, it could be used beside other molecular techniques in differentiation between the salmonella strains.
Keywords
protein; analysis; Comparison; Salmonellae; isolated; Poultry
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