Polymerase chain reaction for differentiation of Pasteurella multocida isolates from turkeys in comparison to strains incorporating in fowl cholera vaccine | ||||
Journal of Veterinary Medical Research | ||||
Article 4, Volume 17, Issue 2, March 2007, Page 21-24 PDF (195.96 K) | ||||
Document Type: Original Article | ||||
DOI: 10.21608/jvmr.2007.77913 | ||||
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Authors | ||||
Nahed I.M.M. Khamis1; Zeinab M. Souror2; Hanan N. Ibrahim2; S. M. Aboul Saoud3 | ||||
1Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt. | ||||
2Central Laboratory for evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt. | ||||
3Veterinary Serum and Vaccine Research Institute, Abbasia, Cairo, Egypt. 2 | ||||
Abstract | ||||
In the present study, polymerase chain reaction (PCR) using random primer (E-20) was used to characterize and identify strains included in this study. Strains included 4 vaccinal reference strains of Pasteurella multocida, CU strain and 4 field isolates of Pasteurella multocida isolated from diseased turkeys which were identified biochemically and serologically as A:1, A:3, A3x4 and D:11. The obtained results revealed that all strains were reacted positively and in different manner with the E20 primer except the 2 field isolates. The results of these reactions demonstrated in terms of bands of different molecular weight specific to each strain. This can be used as a base for characterization and differentiation of strains involved in the present study as the 2 field strains A:1 and A:3 react with primer. Mouse protection test was performed by vaccination of mice with local fowl cholera oil adjuvant vaccine then challenge with virulent field strains A:1, A:3, D:12 and untypable isolates. Results revealed that the local fowl cholera adjuvant vaccine could protect mice against virulent challenge with A:1, A:3 and D:12 field strains but it could not be protect mice against untypable isolates | ||||
Keywords | ||||
Polymerase; chain; reaction; Pasteurella; multocida; isolates; turkeys; Strains; incorporating; vaccine; fowl cholera | ||||
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