Detection of Yersinia enterocolitica in the Shami goat’s milk in the North Sinai Governorate | ||||
| Journal of Veterinary Medical Research | ||||
| Article 29, Volume 15, Issue 2, March 2005, Page 180-183 PDF (160.03 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/jvmr.2005.77966 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Y. M. Kamel; A. L. El-Naggar; H. M. El-Shaer | ||||
| Animal and Poultry Production Division, Desert Research Center, El- Mataria, Cairo, Egypt | ||||
| Abstract | ||||
| Considerable difficulties are associated with the isolation of Yersinia enterocolitica from food particularly milk and milk products. Most methods are time consuming require enrichment steps and are unable to differentiate pathogenic isolates from non pathogenic ones. The purpose of this study was to evaluate the detection of Yersinia enterocolitica in milk by both polymerase chain reaction (PCR) and conventional culturing methods. Fifty milk samples were collected from Shami goats in North Sinai governorate. Two primers (DG26 and DG63) were used in PCR and the size of the PCR-product was 440bp. The results obtained by PCR technique were in good agreement with that obtained by conventional culturing method. Five samples (10%) were positive by PCR while 4 samples were positive by conventional culturing method. Interestingly, PCR results are obtained within few hours. Moreover, it solved the problem of interpretation of classical biochemical and serological typing in one step without necessity of using additional examinations. This makes diagnosis in food control laboratories much faster and more efficient. | ||||
| Keywords | ||||
| Yersinia enterocolitica; the Shami goat’s; Milk | ||||
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