Preparation of diagnostic ELISA kits for detection of camelpox virus
Aboul Soud, E., Sayed, M., Badawi, A., Taha, M., El-Ebiary, E., Daoud, A. (2005). Preparation of diagnostic ELISA kits for detection of camelpox virus. EKB Journal Management System, 15(2), 227-231. doi: 10.21608/jvmr.2005.77992
E. A. Aboul Soud; Magda, M. Sayed; A. A. Badawi; M. M. Taha; Elham A. El-Ebiary; A. M. Daoud. "Preparation of diagnostic ELISA kits for detection of camelpox virus". EKB Journal Management System, 15, 2, 2005, 227-231. doi: 10.21608/jvmr.2005.77992
Aboul Soud, E., Sayed, M., Badawi, A., Taha, M., El-Ebiary, E., Daoud, A. (2005). 'Preparation of diagnostic ELISA kits for detection of camelpox virus', EKB Journal Management System, 15(2), pp. 227-231. doi: 10.21608/jvmr.2005.77992
Aboul Soud, E., Sayed, M., Badawi, A., Taha, M., El-Ebiary, E., Daoud, A. Preparation of diagnostic ELISA kits for detection of camelpox virus. EKB Journal Management System, 2005; 15(2): 227-231. doi: 10.21608/jvmr.2005.77992

Preparation of diagnostic ELISA kits for detection of camelpox virus

Journal of Veterinary Medical Research
Article 37, Volume 15, Issue 2, March 2005, Page 227-231  XML PDF (174.39 K)
Document Type: Original Article
DOI: 10.21608/jvmr.2005.77992
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Authors
E. A. Aboul Soud1; Magda, M. Sayed2; A. A. Badawi2; M. M. Taha1; Elham A. El-Ebiary2; A. M. Daoud1
1Veterinary Serum and Vaccine Research Institute, Abbassia, Cairo, Egypt
2Central Laboratory for Evaluation of Veterinary Biologics, Abbasia, Cairo, Egypt
Abstract
Peroxidase labeled immunoglobulins to camelpox virus (CPV) were prepared for use in various techniques of ELISA. Ten rabbits and three goats were inoculated with a mixture of camelpox virusand Freund’s adjuvant. Sera were pooled separately on the 10th day post the last inoculation and immunoglobulins were precipitated using saturated ammonium sulphate. The globulins were 2.8 g/dl and 2.5 g/dl for rabbits and goats respectively and used for peroxidase conjugation. The peroxidase labeled immunoglobulins were titrated and evaluated using direct solid phase ELISA, double antibody sandwich ELISA and dot immunoblot ELISA. The prepared conjugates gave specific and clear positive reactions till the dilution of 2000 and 1500 for rabbits and goats immunoglobulins respectively. The prepared labeled immunoglobulins could be successfully used in detection of camel pox viral antigen of local virulent and standard vaccinal strain of the virus using various ELISA techniques.
Keywords
ELISA; detection; camelpox virus
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