PRODUCING SUPER MALE (ZZ) AND USING RAPD-PCR TECHNIQUE FOR SEX DIFFERENTIATION IN BLUE TILAPIA (Oreochromis aureus) | ||||
| Zagazig Journal of Agricultural Research | ||||
| Article 8, Volume 40, Issue 4, July 2013, Page 709-722 PDF (1.35 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/zjar.2013.78032 | ||||
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| Abstract | ||||
| A study was made to identify sex differentiation in parents andfries of Oreochromis aureus using RAPD- PCR and squash techniques. Fishes were collected from the experiment shared in program for producing “supermales ZZ” in Egypt. The trial of sex reversal for feminization of sexually undifferentiated progeny was conducted using 17-β ethynylestradiol as a feminizingstimulating agent. Oral administration of powdered feed containing 400 and 800 mg/kg were tested for 30 days in Central Laboratory for Aquaculture Research in Abassa (CLAR).Upon termination of the experiment, average weight and length have increased using high dose. In contrast, the survival rate was increased in low dose (400 mg/kg) than high dose (800 mg/kg). Its effect was highly detectable among fry that were orally fed on 800-mg/kg hormone-treated feeds for 30 days. The mean percentage of phenotypic females was 94%± 2.7 in the second season, females generated progeny including mean percentage of phenotypic males was 69%±4.6 while female, inter sex and non differential percentages were 26% ±3.8, 3%± 1.5 and 2%±0.7 respectively. the sex differentiated have been using applied squash method and RAPD-PCR , Squash method illustrated that from every 10 fishes, 9 individuals were converted into super male (ZZ) . In RAPD -PCR, seven primers were used and they showed different bands patterns between male and female . The total number of DNA fragment band was 35 in maleparents and 33 in femaleparents. While, numbers of DNA fragment bands produced by seven primers were 33 and 35 in adult converted females and fries converted females. Total numbers of DNA fragment bands were 35 and 40 in males and 33 and 35 in female. These molecular markers detected by primers (A14, B13and C05) could be used as markers associated with male or female differentiation. | ||||
| Keywords | ||||
| Oreochromis aureus; sex reversal; feminization and progeny test; RAPD-PCR; squash method | ||||
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