Preparation of buffered acidified plate antigen from Brucella abortus strain 19 | ||||
| Benha Veterinary Medical Journal | ||||
| Article 5, Volume 36, Issue 1, March 2019, Page 43-50 PDF (887.51 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/bvmj.2019.78178 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Lubna Farahat1; Khaled Abd-El-Azeem1; Alaa El Menisy1; Ayman Mahrouse2; Essam A. Nasr.1 | ||||
| 1VSVRI. Abbasia | ||||
| 2Department of Public Health and Zoonoses, General Organization for Veterinary Services. | ||||
| Abstract | ||||
| Brucellosis is one of the major zoonotic infections worldwide, continues to be a public health problem resulting in significant morbidity and economic losses. Accurate diagnosis must include laboratory tests that allow the direct Brucella isolation or indirect detection of antibodies. Practical serological tests are routinely used for the diagnosis of brucellosis by using specific antigens. In the present work, the used buffered acidified plate antigen (BAPA) was prepared from B. abortus biovar 1 strain S1119-3 according to the USDA SOPs. For this purpose, a total of 4100 bovine sera from five farms located in different governorates were screened for brucellosis. The effectiveness of prepared BAPA from strain 19 was compared with the standard BAPA and BCT antigens prepared from B. abortus strain 99. Evaluation was done by using a panel of known dilutions of the OIEISS (Office International des Epizootie International Standard Serum) as the international reference standard serum. There were no significant differences in results of the BAPA antigen prepared from strain 19 and the conventional antigen prepared from strain 99. The results concluded that, the vaccinal strain 19 can be used instead of strain 99 to prepare the BAPA antigen. | ||||
| Keywords | ||||
| Bovine brucellosis; Brucella abortus strain 19; buffered acidified plate agglutination antigen | ||||
|
Statistics Article View: 128 PDF Download: 568 |
||||
