Biological and Molecular Characterization of Newcastle Disease Virus Circulating in Chicken Flocks, Egypt, During 2014-2015
Abd El Aziz, M., Abd El-Hamid, H., Ellkany, H., Nasef, S., Nasr, S., El Bestawy, A. (2016). Biological and Molecular Characterization of Newcastle Disease Virus Circulating in Chicken Flocks, Egypt, During 2014-2015. EKB Journal Management System, 44(1), 9-20. doi: 10.21608/zvjz.2016.7827
Mohamed Abd El Aziz; Hatem Abd El-Hamid; Hany Ellkany; Soad Nasef; Sherif Nasr; Ahmed El Bestawy. "Biological and Molecular Characterization of Newcastle Disease Virus Circulating in Chicken Flocks, Egypt, During 2014-2015". EKB Journal Management System, 44, 1, 2016, 9-20. doi: 10.21608/zvjz.2016.7827
Abd El Aziz, M., Abd El-Hamid, H., Ellkany, H., Nasef, S., Nasr, S., El Bestawy, A. (2016). 'Biological and Molecular Characterization of Newcastle Disease Virus Circulating in Chicken Flocks, Egypt, During 2014-2015', EKB Journal Management System, 44(1), pp. 9-20. doi: 10.21608/zvjz.2016.7827
Abd El Aziz, M., Abd El-Hamid, H., Ellkany, H., Nasef, S., Nasr, S., El Bestawy, A. Biological and Molecular Characterization of Newcastle Disease Virus Circulating in Chicken Flocks, Egypt, During 2014-2015. EKB Journal Management System, 2016; 44(1): 9-20. doi: 10.21608/zvjz.2016.7827

Biological and Molecular Characterization of Newcastle Disease Virus Circulating in Chicken Flocks, Egypt, During 2014-2015

Zagazig Veterinary Journal
Article 2, Volume 44, Issue 1, March 2016, Page 9-20  XML PDF (674.51 K)
Document Type: Original Article
DOI: 10.21608/zvjz.2016.7827
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Authors
Mohamed Abd El Aziz email 1; Hatem Abd El-Hamid1; Hany Ellkany1; Soad Nasef2; Sherif Nasr3; Ahmed El Bestawy1
1Poultry and Fish Diseases Department, Faculty of Veterinary Medicine, Damanhur University, Damanhur, Egypt
2Animal Health Research Institute, National Lab. of Vet. Quality Control On Poultry Production (NLQP), Dokki, Giza, Egypt
3Animal Husbandry and Animal Wealth Development Department, Faculty of Veterinary Medicine, Damanhur University, Damanhur, Egypt
Abstract
 
Newcastle disease (ND), a highly contagious disease, is responsible for disturbing disease outbreaks in poultry flocks worldwide. This study was carried out to isolate and characterize the circulating Newcastle Disease Virus (NDV) strains in different chicken flocks (broilers, commercial egg layers and breeders) in four Egyptian governorates (Gharbia, El Behera, Dakahlia and Kafer EL Shiekh) during 2014 and 2015. Forty-eight suspected NDV infected flocks were examined clinically and used for virus isolation and characterization. The mortality rates among the examined flocks were 5-85 %. The deduced amino acid sequences of F protein gene revealed that four NDV isolates possessed the motif 112 R/K-R-Q-K/RR116at the C-terminus of the F2 protein and F (phenylalanine) at residue 117, indicating that they are velogenic genotype. The present study revealed the circulation of NDV class II genotype VII in Egyptian chicken flocks.
Keywords
Newcastle disease; genotype VII; F protein
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