Phenotypic and genotypic characterization of S. aureus isolated from clinical and subclinical bovine mastitis
Enany, M., Younes, S., AL gammal, A., Salem, M., El Dieb, H. (2013). Phenotypic and genotypic characterization of S. aureus isolated from clinical and subclinical bovine mastitis. EKB Journal Management System, 18(1), 139-147. doi: 10.21608/scvmj.2013.78287
Mohamed Enany; Soha Younes; Abd Elazeem AL gammal; Mohamed Salem; Hoda El Dieb. "Phenotypic and genotypic characterization of S. aureus isolated from clinical and subclinical bovine mastitis". EKB Journal Management System, 18, 1, 2013, 139-147. doi: 10.21608/scvmj.2013.78287
Enany, M., Younes, S., AL gammal, A., Salem, M., El Dieb, H. (2013). 'Phenotypic and genotypic characterization of S. aureus isolated from clinical and subclinical bovine mastitis', EKB Journal Management System, 18(1), pp. 139-147. doi: 10.21608/scvmj.2013.78287
Enany, M., Younes, S., AL gammal, A., Salem, M., El Dieb, H. Phenotypic and genotypic characterization of S. aureus isolated from clinical and subclinical bovine mastitis. EKB Journal Management System, 2013; 18(1): 139-147. doi: 10.21608/scvmj.2013.78287

Phenotypic and genotypic characterization of S. aureus isolated from clinical and subclinical bovine mastitis

Suez Canal Veterinary Medical Journal. SCVMJ
Article 12, Volume 18, Issue 1, June 2013, Page 139-147  XML PDF (330.25 K)
Document Type: Original Article
DOI: 10.21608/scvmj.2013.78287
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Authors
Mohamed Enany1; Soha Younes2; Abd Elazeem AL gammal1; Mohamed Salem3; Hoda El Dieb* 1
1Department of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University
2Department of Clinical pathology, Faculty of Medicine, Suez Canal University
3Department of Animal Medicine, Faculty of Veterinary Medicine, Suez Canal University
Abstract
In order to determine the prevalence of S. aureus in clinical and subclinical bovine mastitis as well as the relationship between the presence of protein A gene and the udder inflammatory response .   A total of 400 quarter milk samples were collected from 100 lactating Friesian cows  at Ismailia Governorate . The percentage of clinical mastitis was   ( 19.5%.), while the percentage of subclinical mastitis according to C.M.T was ( 42.85%) . The percentage of  S.aureus   in clinical  mastitis was (28.2%) , while in  in subclinical mastitis  was (22.46% ).The identification of S,aureus was confirmed by PCR amplification of species-specific parts of the gene encoding the 16S rRNA. Twenty nine  S.aureus isolates were subjected to PCR for detection of X region of protein A (spA) gene,10 isolates from clinical mastitis and 19  isolates subclinical mastitiC milk specimens (+++ve CMT and ++ve CMT)  were (100%) positive . Results indicate that there  was a positive relationship between the  presence of protein A gene of S.aureus and the inflammation of udder in clinical mastitis as well as the high grades of subclinical mastitis. 
Keywords
Phenotypic; genotypic; S. aureus; bovine; mastitis
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