Phenetic and Phylogenetic Relationships of Egyptian Hordeum vulgare L. Using Agro-morphological Criteria, Molecular Marker and Genes Expression
Osman, S., Mostafa, E., El-Ashry, Z., Mohamed, F., Elatroush, H., El-Khodary, S. (2020). Phenetic and Phylogenetic Relationships of Egyptian Hordeum vulgare L. Using Agro-morphological Criteria, Molecular Marker and Genes Expression. EKB Journal Management System, 40(1), 27-40. doi: 10.21608/taec.2020.23618.1015
Samira Ali Hassan Osman; Essam Ahmed Mostafa; Zeinab Mohamed El-Ashry; Fawzia Ismail Mohamed; Hala Elatroush; Soheir El-Khodary. "Phenetic and Phylogenetic Relationships of Egyptian Hordeum vulgare L. Using Agro-morphological Criteria, Molecular Marker and Genes Expression". EKB Journal Management System, 40, 1, 2020, 27-40. doi: 10.21608/taec.2020.23618.1015
Osman, S., Mostafa, E., El-Ashry, Z., Mohamed, F., Elatroush, H., El-Khodary, S. (2020). 'Phenetic and Phylogenetic Relationships of Egyptian Hordeum vulgare L. Using Agro-morphological Criteria, Molecular Marker and Genes Expression', EKB Journal Management System, 40(1), pp. 27-40. doi: 10.21608/taec.2020.23618.1015
Osman, S., Mostafa, E., El-Ashry, Z., Mohamed, F., Elatroush, H., El-Khodary, S. Phenetic and Phylogenetic Relationships of Egyptian Hordeum vulgare L. Using Agro-morphological Criteria, Molecular Marker and Genes Expression. EKB Journal Management System, 2020; 40(1): 27-40. doi: 10.21608/taec.2020.23618.1015

Phenetic and Phylogenetic Relationships of Egyptian Hordeum vulgare L. Using Agro-morphological Criteria, Molecular Marker and Genes Expression

Taeckholmia
Article 3, Volume 40, Issue 1, 2020, Page 27-40  XML PDF (906.26 K)
Document Type: Original Article
DOI: 10.21608/taec.2020.23618.1015
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Authors
Samira Ali Hassan Osman email orcid 1; Essam Ahmed Mostafa2; Zeinab Mohamed El-Ashry3; Fawzia Ismail Mohamed3; Hala Elatroush4; Soheir El-Khodary5
1Genetics and Cytology dept., Genetic Engineering and Biotechnology dev., National Research Centre, Giza, Egypt
2Genetics and Cytology dept., National Research Centre, Giza, Egypt
3Genetics and Cytology dept., National Research Centre, Giza, Egypt.
4Botany Department, Faculty of Science, Ain Shams University, Cairo
5Botany dept., faculty of Science, Ain Shams University.
Abstract
Phenetic and phylogenetic relationships between ten Hordeum vulgare L. taxa , seven cultivars (Giza 123, Giza 126, Giza 127, Giza 128, Giza 129, Giza 130 and Giza 2000) and three landraces collected from Sinai (El-Kheroba, El-Sheikh Zuwaid and Wadi Sedr). This study was carried out using morphological criteria, molecular genetic markers (SSRs) and finally soluble protein patterns. The results of morphological traits reflected that Giza 2000 and Wadi Sedr gave superior in vegetative growth and yield productivity in contrast to Giza 129, which showed an inferior vegetative growth and yield. The SSR analyses using 7 primers and revealed detection of a total of 15 bands, among which 8 bands (61.22%) were polymorphic. Finally we can conclude that, the genetic distance tree was produced by UPGMA based on morphological data stated the pedigree of studied cultivars. However, in the tree based on molecular markers (SSRs) and soluble proteins patterns were not in full agreement with the pedigree.
Keywords
Hordeum vulgare L; genetic relationships; morphological Criteria; SDS-PAGE Electrophoresis; Molecular markers (SSR-PCR)
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