Impact of omega-3 fatty acids on evolution of carbon tetrachloride- (CCl4) induced liver cirrhosis in mice : A histological and immunohistochemical study
Abu-Dief, E., Mohammed, D., Abd El-Haliem, N., Mohammed, S., El-Badry, A. (2018). Impact of omega-3 fatty acids on evolution of carbon tetrachloride- (CCl4) induced liver cirrhosis in mice : A histological and immunohistochemical study. EKB Journal Management System, 41(1), 61-72. doi: 10.21608/EJH.2018.7522
Eman Abu-Dief; Doha Mohammed; Nesreen Abd El-Haliem; Sherine Mohammed; Ashraf M. El-Badry. "Impact of omega-3 fatty acids on evolution of carbon tetrachloride- (CCl4) induced liver cirrhosis in mice : A histological and immunohistochemical study". EKB Journal Management System, 41, 1, 2018, 61-72. doi: 10.21608/EJH.2018.7522
Abu-Dief, E., Mohammed, D., Abd El-Haliem, N., Mohammed, S., El-Badry, A. (2018). 'Impact of omega-3 fatty acids on evolution of carbon tetrachloride- (CCl4) induced liver cirrhosis in mice : A histological and immunohistochemical study', EKB Journal Management System, 41(1), pp. 61-72. doi: 10.21608/EJH.2018.7522
Abu-Dief, E., Mohammed, D., Abd El-Haliem, N., Mohammed, S., El-Badry, A. Impact of omega-3 fatty acids on evolution of carbon tetrachloride- (CCl4) induced liver cirrhosis in mice : A histological and immunohistochemical study. EKB Journal Management System, 2018; 41(1): 61-72. doi: 10.21608/EJH.2018.7522

Impact of omega-3 fatty acids on evolution of carbon tetrachloride- (CCl4) induced liver cirrhosis in mice : A histological and immunohistochemical study

Egyptian Journal of Histology
Article 6, Volume 41, Issue 1, March 2018, Page 61-72  XML PDF (2.86 MB)
Document Type: Original Article
DOI: 10.21608/EJH.2018.7522
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Authors
Eman Abu-Dief1; Doha Mohammed1; Nesreen Abd El-Haliem1; Sherine Mohammed* 1; Ashraf M. El-Badry2
1Department of Histology, Faculty of Medicine, Sohag University, Sohag, Egypt
2Department of Surgery, Faculty of Medicine, Sohag University, Sohag, Egypt
Abstract
hepatic architecture. Omega-3 polyunsaturated fatty acids are common antioxidant, anti-inflammatory and anti-fibrotic agents
Aim: This study was conducted to investigate the potential effects of omega-3 fatty acids on experimentally induced cirrhosis.
Materials and Methods: 60 animals were divided into three groups: Group I (control): 10 animals, group II: 25 animals treated with carbon tetrachloride (CCl4), and Group III : 25 animals treated with CCl4 then with omega-3 concomitant with CCl4 for additional two weeks after induction of cirrhosis. Body weight and liver weight coefficient were measured. Liver samples were taken for histological and immunohistochemical studies.
Results: Omega-3 fatty acids treatment attenuated the CCl4 induced cirrhosis. Histological studies revealed that it decreased the stage of fibrosis, and the grade of the necroinflammatory changes. Immunohistochemical studies demonstrated that apoptotic changes diminished in cirrhotic livers after treatment with omega-3 fatty acids with a significant decrease in the cleaved caspase-3 index. Furthermore, there was a significant decrease in the number of macrophages, and hepatic stellate cells activation.
Conclusion: Our results suggest that omega-3 fatty acids have an anti-inflammatory and anti-fibrotic effects on CCl4 induced liver cirrhosis in mice.
Keywords
apoptosis; α-SMA; fibrosis; Liver; omega-3; TIMP-1
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