Zoonotic Importance of Some Pathogenic Bacteria Isolated from Small Ruminants' Milk and Hands of Dairy Workers
El-Mahallawy, H., Elhariri, M., Elhelw, R., Hamza, D. (2017). Zoonotic Importance of Some Pathogenic Bacteria Isolated from Small Ruminants' Milk and Hands of Dairy Workers. EKB Journal Management System, 45(4), 305-313. doi: 10.21608/zvjz.2017.7861
Heba S. El-Mahallawy; Mahmoud Elhariri; Rehab Elhelw; Dalia Hamza. "Zoonotic Importance of Some Pathogenic Bacteria Isolated from Small Ruminants' Milk and Hands of Dairy Workers". EKB Journal Management System, 45, 4, 2017, 305-313. doi: 10.21608/zvjz.2017.7861
El-Mahallawy, H., Elhariri, M., Elhelw, R., Hamza, D. (2017). 'Zoonotic Importance of Some Pathogenic Bacteria Isolated from Small Ruminants' Milk and Hands of Dairy Workers', EKB Journal Management System, 45(4), pp. 305-313. doi: 10.21608/zvjz.2017.7861
El-Mahallawy, H., Elhariri, M., Elhelw, R., Hamza, D. Zoonotic Importance of Some Pathogenic Bacteria Isolated from Small Ruminants' Milk and Hands of Dairy Workers. EKB Journal Management System, 2017; 45(4): 305-313. doi: 10.21608/zvjz.2017.7861

Zoonotic Importance of Some Pathogenic Bacteria Isolated from Small Ruminants' Milk and Hands of Dairy Workers

Zagazig Veterinary Journal
Article 1, Volume 45, Issue 4, December 2017, Page 305-313  XML PDF (267.44 K)
Document Type: Original Article
DOI: 10.21608/zvjz.2017.7861
View on SCiNiTO View on SCiNiTO
Authors
Heba S. El-Mahallawy email 1; Mahmoud Elhariri2; Rehab Elhelw2; Dalia Hamza3
1Animal Hygiene, Zoonoses and Animal Behaviour and Management Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt
2Microbiology Department, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt
3Zoonoses Department, Faculty of Veterinary Medicine, Cairo University, Cairo, Egypt
Abstract
Staphylococcus aureus and enteropathogenic Escherichia coli are among zoonotic bacterial food-borne pathogens causing illness ranged from diarrhoea to fatal conditions. This study was undertaken to evaluate the occurrence of Staph. aureus and E. coli in small ruminants' raw milk and hands of dairy workers at small-scale production units, Giza, Egypt. A total of 420 raw milk samples were obtained from apparently healthy sheep and goats, also, hand swabs (n=46) from workers at the units under investigation were examined. Overall, Staph. aureus (13.1%, 55/420) and E. coli (26.2%, 110/420) isolates were obtained from raw milk of sheep and goats. However, only Staph. aureus (15.2%, 7/46) was recovered from hand swabs of dairy workers. Using PCR, all the tested Staph. aureus isolates from milk and hand swabs yielded specific bands of Thermonuclease (nuc) gene at 279 bp, however, amplification of virulence (eae) gene encoding the intimin protein of E. coli produced an amplicon of 917 bp in 45% of the tested E. coli isolates. In conclusion, our findings provide an overview about Staph. aureus and E. colicontamination in raw milk of small ruminants locally bred by smallholders and suggest probably transmission of Staph. aureus from hands of dairy workers that contaminate the milk or udder of apparently healthy animals. Reporting of such organisms from milk indicates the need for strict hygienic measures during milking at these production units.
 
Keywords
Raw milk; Dairy workers; sheep; Goat; E. coli; Staph. aureus
Statistics
Article View: 247
PDF Download: 384