Quantification of osteocyte apoptosis during experimental tooth movement as revealed by immunohistochemistry of Caspase 3
Kassem, H., Talaat, I., El Sawa, A., Ismail, H., Zaher, A. (2016). Quantification of osteocyte apoptosis during experimental tooth movement as revealed by immunohistochemistry of Caspase 3. EKB Journal Management System, 49(June 2016), 1-10. doi: 10.21608/eos.2016.78681
Hassan Kassem; Iman Talaat; Afaf El Sawa; hanan Ismail; Abbas Zaher. "Quantification of osteocyte apoptosis during experimental tooth movement as revealed by immunohistochemistry of Caspase 3". EKB Journal Management System, 49, June 2016, 2016, 1-10. doi: 10.21608/eos.2016.78681
Kassem, H., Talaat, I., El Sawa, A., Ismail, H., Zaher, A. (2016). 'Quantification of osteocyte apoptosis during experimental tooth movement as revealed by immunohistochemistry of Caspase 3', EKB Journal Management System, 49(June 2016), pp. 1-10. doi: 10.21608/eos.2016.78681
Kassem, H., Talaat, I., El Sawa, A., Ismail, H., Zaher, A. Quantification of osteocyte apoptosis during experimental tooth movement as revealed by immunohistochemistry of Caspase 3. EKB Journal Management System, 2016; 49(June 2016): 1-10. doi: 10.21608/eos.2016.78681

Quantification of osteocyte apoptosis during experimental tooth movement as revealed by immunohistochemistry of Caspase 3

Egyptian Orthodontic Journal
Article 1, Volume 49, June 2016, June 2016, Page 1-10  XML PDF (588.58 K)
Document Type: Original Article
DOI: 10.21608/eos.2016.78681
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Authors
Hassan Kassem* 1; Iman Talaat2; Afaf El Sawa3; hanan Ismail1; Abbas Zaher1
1Department of Orthodontics, Faculty of Dentistry, Alexandria University.
2Department of Pathology, Faculty of Medicine, Alexandria University
3Department of Oral Biology, Faculty of Dentistry, Alexandria Universit
Abstract
 
 Objective: To investigate the time course of osteocyte apoptosis in a rat model of orthodontic tooth movement as revealed by the caspase-3 activation pathway. Materials and Methods: Fifteen male Sprague Dawley rats (6–9weeks old) were loaded with an orthodontic appliance. A spring delivering 10–12 g of force was placed between the maxillary first molar and the incisor to displace the first molar mesially. The animals were equally divided into three time points: 24 hours, 3 and 7 days of orthodontic loading. Five animals served as 0 day group. Caspase 3 immunostaining, was performed on histologic sections of the first molars. The labeling was quantified in osteocytes on the mesial and distal sides of the alveolar bone in each group. Results: Caspase 3 labeling at the mesial side significantly increased at 1, 3 and 7 days after orthodontic loading compared to the control group. No peak increase was observed. There was no significant difference in caspase 3 labeling at the distal side. Conclusions: Osteocyte apoptosis during orthodontic tooth movement could be revealed by caspase 3 immunostaining. This suggest that caspase 3 apoptotic pathway is involved in osteocyte death during orthodontic tooth movement.
Keywords
Osteocyte; apoptosis; Caspase 3; orthodontic tooth movement
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