Effects of dexamethasone , adipose derived stem cells and their exosomes on inflammation and oral wound healing: Comparative histo logic study in rats | ||||
| Egyptian Dental Journal | ||||
| Article 10, Volume 64, Issue 4 - October (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), October 2018, Page 3367-3377 PDF (3.48 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/edj.2018.78904 | ||||
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| Authors | ||||
| Ebtehal Mohammed1; Eman Khalil2; Dina Sabry3 | ||||
| 1Lecturer of Oral Medicine , Oral diagnosis and Periodontology , Faculty of Dentistry, Beni Suef University. | ||||
| 2Lecturer of Oral Medicine, Oral diagnosis and Periodontology , Faculty of Dentistry, British University. Egypt. | ||||
| 3Professor of Biochemistry, Faculty of Medicine, Cairo University. 11562. Cairo. Egypt. | ||||
| Abstract | ||||
| The inflammatory phase of wound healing affects the wound repair as down regulation or up regulation result in delayed healing or scar formation. The dexamethasone is the drug of choice as anti inflammatory, anti edematous and anti emetic in different oral and periodontal surgeries to control post operative pain and edema, however the down regulation of inflammatory phase may affect the wound healing. Adipose derived stem cells (ADSCs), as a part of cell therapy, and exosomes (Exo.) , as a part of cell free therapy, revealed promising results in wound healing in preclinical and clinical studies. Materials and Methods: 5o rats were involved in this study. The standardized oral wound was done by scalpel in left cheek then the animals were randomly assigned to 4 groups including gelatin sponge group, dexamethasone group, Exo. group and ADSCs group. The animals were sacrificed at 2,5 and 10 days of healing. The specimens were evaluated histologically (H&E) stains and inflammatory cells were counted and statistically compared. Results: regarding inflammatory cell counting, the dexamethasone revealed least inflammatory infiltration followed by Exo.group then ADSCs group and finally, gelatin sponge group. All groups showed signs of healing, However the Exo. group followed by ADSCs showed more condensed collagen formation and increased epithelial thickness than dexamethasone and gelatin sponge groups. Conclusion: Exo. and ADSCs may accelerate the oral wound healing and overcome the delayed healing that may limit the use of dexamethasone administration as anti inflammatory drug, especially in medically comprised patients as diabetic patients. | ||||
| Keywords | ||||
| Oral wound healing; dexamethasone; adipose derived stem cells; exosomes | ||||
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