Comparative study of the cytoto xicity and apoptotic effect of beet root, and silica-beet nanopartic les with 5-FU nanopartic les against Scc-090 cell line
Zayed, S., Hamed, R., El Dakrory, U. (2018). Comparative study of the cytoto xicity and apoptotic effect of beet root, and silica-beet nanopartic les with 5-FU nanopartic les against Scc-090 cell line. EKB Journal Management System, 64(Issue 4 - October (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)), 3379-3389. doi: 10.21608/edj.2018.79054
Shaimaa Omar Zayed; Reham S. Hamed; Usama Abd El Rouf El Dakrory. "Comparative study of the cytoto xicity and apoptotic effect of beet root, and silica-beet nanopartic les with 5-FU nanopartic les against Scc-090 cell line". EKB Journal Management System, 64, Issue 4 - October (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), 2018, 3379-3389. doi: 10.21608/edj.2018.79054
Zayed, S., Hamed, R., El Dakrory, U. (2018). 'Comparative study of the cytoto xicity and apoptotic effect of beet root, and silica-beet nanopartic les with 5-FU nanopartic les against Scc-090 cell line', EKB Journal Management System, 64(Issue 4 - October (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)), pp. 3379-3389. doi: 10.21608/edj.2018.79054
Zayed, S., Hamed, R., El Dakrory, U. Comparative study of the cytoto xicity and apoptotic effect of beet root, and silica-beet nanopartic les with 5-FU nanopartic les against Scc-090 cell line. EKB Journal Management System, 2018; 64(Issue 4 - October (Oral Medicine, X-Ray, Oral Biology & Oral Pathology)): 3379-3389. doi: 10.21608/edj.2018.79054

Comparative study of the cytoto xicity and apoptotic effect of beet root, and silica-beet nanopartic les with 5-FU nanopartic les against Scc-090 cell line

Egyptian Dental Journal
Article 11, Volume 64, Issue 4 - October (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), October 2018, Page 3379-3389  XML PDF (675.99 K)
Document Type: Original Article
DOI: 10.21608/edj.2018.79054
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Authors
Shaimaa Omar Zayed1; Reham S. Hamed2; Usama Abd El Rouf El Dakrory3
1Lecturer, Oral Pathology Department, Faculty of Oral and Dental Medicine, Cairo University
2Lecturer, Oral surgery Department, Faculty of Oral surgey, Misr University for science and technology
3Lecturer, Oral surgery Department, Faculty of Oral surgey, Misr University for science and technology.
Abstract
Objective: Nanoparticle delivery systems can be used to target anticancer drugs to tumor
tissues. Beet root appears to be a powerful dietary source of antioxidant agent with anti-cancer
effect due to ability to scavenge free radicals. Application of silica-nanoparticles (SiNP) in cancer
treatment are promising, with increased data suggesting anti-proliferative effects in cancer cells.
The aim of our study was to compare the cytotoxic effect of the beet root NP, silica-beet NP with
5fu (flourarcil) NP , a chemotherapeutic agent against tongue carcinoma cell line scc-090.
Methods: Cell Line cells were obtained from American Type Culture Collection , cells were
cultured using DMEM (Invitrogen/Life Technologies). Beet root nanoparticles Si-beet NP and 5FU
drug NP were used. The effect of the tested materials were investigated by MTTassay and DNA
fragmentation (DPA method).
Results: The MTT cytotoxicity assay revealed that beat root (nano) and Si-beat roots (nano)
have obbvious cytotoxicity on SS C-090 cancer cell lines at low significant IC50 value as 0.73μg/
ml and 2.94 μg/ml respectively. While 5FU drug revealed obvious cytotoxicity on SSC-090 cancer
cell lines at higher value IC50 8.83 ug/ml. these results denote that low doses of beat root (nano)
may exert remarkable cell death on tongue cancer cells line scc-090.
Conclusion: Our findings demonstrate that beet root NP could be capable of inducing cancer
cell death with prominent apoptosis at very low ic50 value 0.73μg/ml against oral tongue carcinoma
SCC-090 cell lines.
 
Keywords
Beet root; Silica nanoparticles; 5-FU drug and Apoptosis
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