Effect of Bone Marrow- Derived Stem Cells on Apopto sis of Dental Pulp Cells of Strepto zotocin -Induced Diabetic Rats | ||||
| Egyptian Dental Journal | ||||
| Article 15, Volume 64, Issue 4 - October (Oral Medicine, X-Ray, Oral Biology & Oral Pathology), October 2018, Page 3429-3437 PDF (1.45 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/edj.2018.79100 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
| Mervat M. Youssef1; Wafaa El-Hossary2; Heba A. Elsaied3 | ||||
| 1Assistant professor of Oral Biology, Faculty Of Dentistry, Suez Canal University | ||||
| 2Lecturer of Oral Pathology, Faculty Of Dentistry, Suez Canal University | ||||
| 3Lecturer of Oral Biology, Faculty Of Dentistry, Suez Canal University. | ||||
| Abstract | ||||
| Aim: the aim of the present investigation was to study the effect of mesenchymal bone marrow stem cells (BMSCs) on the histological structure of the dental pulp of rats with Streptozotocininduced diabetes mellitus using hematoxylin & eosin and Masson’s trichrome stains. Furthermore, the apoptotic activity of dental pulp cells was detected through Caspase-9 localization. Material and Methods: Thirty, three- month old, adult male albino rats, 180-200 gram weight were used in this investigation. They were divided into three groups. Group I: 10 animals served as controls. Group II: 10 animals subjected to a single intraperitoneal injection of Streptozotocin in a dose of 60 mg/kg body weight for induction of diabetes mellitus that was verified by detecting hyperglycaemia (200mg/dl or more) in a blood sample. Group III: 10 animals treated the same as those of group II and one week later these animals were intravenously injected with BMSCs. At the end of the experiment which lasted for two months, the animals of the different groups were euthanized. Their jaws were dissected, decalcified and processed for hematoxylin and eosin, Masson’s trichrome stains and immunohistochemical stain for caspase-9 to study the dental pulp of the experimental rats. Results: The present work revealed that the treatment of diabetic rats with single intravenous injection of bone marrow stem cells resulted in amelioration of the degenerative and apoptotic .effect of diabetes on the pulpal tissues Conclusion: Induction of diabetes in albino rats has degenerative effect on pulpal tissues accompanied by increased expression of caspase-9 (apoptotic marker). Intravenous injection of BMSCs resulted in regeneration of pulp tissues and decreased apoptosis as detected by low caspase-9 expression. | ||||
| Keywords | ||||
| stem cells; apoptosis; dental pulp; diabetes; caspase-9 | ||||
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