Insecticidal Activity of Radish, Raphanus sativus Linn. (Brassicaceae) Roots Extracts.
Ibrahim, H., Abdel-Mogib, M., Mostafa, M. (2020). Insecticidal Activity of Radish, Raphanus sativus Linn. (Brassicaceae) Roots Extracts.. EKB Journal Management System, 11(1), 53-58. doi: 10.21608/jppp.2020.79164
Heba Y. E. Ibrahim; M. Abdel-Mogib; M. El. Mostafa. "Insecticidal Activity of Radish, Raphanus sativus Linn. (Brassicaceae) Roots Extracts.". EKB Journal Management System, 11, 1, 2020, 53-58. doi: 10.21608/jppp.2020.79164
Ibrahim, H., Abdel-Mogib, M., Mostafa, M. (2020). 'Insecticidal Activity of Radish, Raphanus sativus Linn. (Brassicaceae) Roots Extracts.', EKB Journal Management System, 11(1), pp. 53-58. doi: 10.21608/jppp.2020.79164
Ibrahim, H., Abdel-Mogib, M., Mostafa, M. Insecticidal Activity of Radish, Raphanus sativus Linn. (Brassicaceae) Roots Extracts.. EKB Journal Management System, 2020; 11(1): 53-58. doi: 10.21608/jppp.2020.79164

Insecticidal Activity of Radish, Raphanus sativus Linn. (Brassicaceae) Roots Extracts.

Journal of Plant Protection and Pathology
Article 11, Volume 11, Issue 1, January 2020, Page 53-58  XML PDF (376.88 K)
Document Type: Original Article
DOI: 10.21608/jppp.2020.79164
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Authors
Heba Y. E. Ibrahim email 1; M. Abdel-Mogib2; M. El. Mostafa1
1Plant Protection Research Institute, Agricultural Research Center (ARC), Dokki, Giza, 12618, Egypt.
2Chemistry Department, Faculty of Science, Mansoura University, Mansoura, 35516, Egypt
Abstract
Chemical constituents of Raphanus sativus Linn. roots were extracted and tested for their toxicity against adults and second instar nymphs of A. gossypii under laboratory conditions. The most effective fraction against both adults and nymphs was methylene chloride fraction with LC50 values of 386.63 and 309.43 ppm, followed by ethyl acetate fraction with LC50 values of 394.9 and 334.37 ppm then petroleum ether fraction with LC50 values of 636.2 and 424.56 ppm, for adults and nymphs, respectively. Also, the volatile components of each fraction were qualitatively and quantitatively identified and characterized by GC/MS technique. Moreover, the impact of the sub-lethal concentrations of R. sativus roots extracts on transaminases and alkaline phosphatase of A. gossypii was studied. Great inhibition of all tested enzymes activity was observed.  This suggested the high potency of R. sativus roots extracts as environmentally friendly alternatives of traditional insecticides.
Chemical constituents of Raphanus sativus Linn. roots were extracted and tested for their toxicity against adults and second instar nymphs of A. gossypii under laboratory conditions. The most effective fraction against both adults and nymphs was methylene chloride fraction with LC50 values of 386.63 and 309.43 ppm, followed by ethyl acetate fraction with LC50 values of 394.9 and 334.37 ppm then petroleum ether fraction with LC50 values of 636.2 and 424.56 ppm, for adults and nymphs, respectively. Also, the volatile components of each fraction were qualitatively and quantitatively identified and characterized by GC/MS technique. Moreover, the impact of the sub-lethal concentrations of R. sativus roots extracts on transaminases and alkaline phosphatase of A. gossypii was studied. Great inhibition of all tested enzymes activity was observed.  This suggested the high potency of R. sativus roots extracts as environmentally friendly alternatives of traditional insecticides.
 
Keywords
Raphanus sativus; insecticidal activity; Aphis gossypii; transaminases and alkaline phosphatase
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