Isolation of Different Clostridium Isolates for Acetone-Butanol-Ethanol (ABE) Production from Sargassum sp.
Mansour, H., Madian, H., Farahat, L., Hoballah, E., Ibrahim, M. (2020). Isolation of Different Clostridium Isolates for Acetone-Butanol-Ethanol (ABE) Production from Sargassum sp.. EKB Journal Management System, 63(11), 4261-4272. doi: 10.21608/ejchem.2020.24250.2448
Heba I. Mansour; Hekmat R. Madian; Laila A. Farahat; Essam A. Hoballah; Mohamed K. Ibrahim. "Isolation of Different Clostridium Isolates for Acetone-Butanol-Ethanol (ABE) Production from Sargassum sp.". EKB Journal Management System, 63, 11, 2020, 4261-4272. doi: 10.21608/ejchem.2020.24250.2448
Mansour, H., Madian, H., Farahat, L., Hoballah, E., Ibrahim, M. (2020). 'Isolation of Different Clostridium Isolates for Acetone-Butanol-Ethanol (ABE) Production from Sargassum sp.', EKB Journal Management System, 63(11), pp. 4261-4272. doi: 10.21608/ejchem.2020.24250.2448
Mansour, H., Madian, H., Farahat, L., Hoballah, E., Ibrahim, M. Isolation of Different Clostridium Isolates for Acetone-Butanol-Ethanol (ABE) Production from Sargassum sp.. EKB Journal Management System, 2020; 63(11): 4261-4272. doi: 10.21608/ejchem.2020.24250.2448

Isolation of Different Clostridium Isolates for Acetone-Butanol-Ethanol (ABE) Production from Sargassum sp.

Egyptian Journal of Chemistry
Article 11, Volume 63, Issue 11, November 2020, Page 4261-4272  XML PDF (458.07 K)
Document Type: Original Article
DOI: 10.21608/ejchem.2020.24250.2448
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Authors
Heba I. Mansour email 1; Hekmat R. Madian1; Laila A. Farahat1; Essam A. Hoballah2; Mohamed K. Ibrahim3
1Process design and Development Department, Egyptian Petroleum Research Institute (EPRI), Egypt
2Environmental Microbiology Department, National Research Center, Egypt
3Microbiology Department, Faculty of Science, Ain Shams University, Egypt
Abstract
Macroalgae with several species are an abundant, carbon-neutral renewable resource and rich in carbohydrates which make it suitable for biobutanol production. Recently, due to advantages of biobutanol as a liquid biofuel, it can be used as a substitute for gasoline and diesel. Many researches mentioned that, different Clostridium species are able to use fermentable sugars for production of biobutanol from different biomasses through ABE (Acetone- Butanol- Ethanol) fermentation process. In this study the (Sargassum sp.) was used as carbon source for biobutanol production. Thirty-three anaerobic mesophilic isolates were isolated on RCM medium from five soil cultivated with different crops. Only thirteen spore forming, mesophilic, anaerobic Clostridium isolates used for the fermentation process. 100 g/L from Sargassum sp were hydrolyzed by sulfuric acid (6 % (v/v)) followed by thermal pretreatment at 121°C for 20 minutes to produce total reducing sugars (24.151 ± 0.273 g/L), which were fermented by Clostridium isolates. The most promising isolate (HG1) which produce the highest butanol level, acetone and ethanol (3.743, 1.401 and 1.031 g/l) respectively was identified according to Butanol dehydrogenases (bdhA) gene analysis as Clostridium acetobutylicum. This paper emphasizes the importance of (Sargassum sp.) as a renewable feedstock for the biobutanol production.
Keywords
Macroalgae. Sargassum sp; Acid hydrolysis; Clostridium. Isolation; ABE fermentation; Biobutanol
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