Plasma Circular RNA (0054633) Expression as a Biomarker for Prediabetes and Type 2 diabetes mellitus | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 7, Volume 38, Issue 1, January 2022, Page 77-88 PDF (693.82 K) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2018.8113 | ||||
 View on SCiNiTO | ||||
| Authors | ||||
Sally El-Hefnway   1; Nevein Al-sheikh1; Heba Kasem2; Fatma El-Esrigy3
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| 1Medical Biochemistry Department, Faculty of Medicine, Menoufia University, Egypt | ||||
| 2Internal Medicine Department, Faculty of Medicine, Menoufia University, Egypt. | ||||
| 3Family Medicine Department, Faculty of Medicine, Menoufia University, Egypt. | ||||
| Abstract | ||||
| Background: Circular RNAs are a type of closed non-coding RNAs, lack the terminating 5'-cap and 3'-polyadenylated tail structures that present in linear RNAs. They have higher biological stability because of their resistance to RNA exonucleases and in some tissues; their expression levels are ten times higher than other types of RNA. CircRNAs act as biomarkers for various diseases including diabetes mellitus as they have been found to affect insulin secretion and β-cell renewal. Objective: to investigate the role of circRNA (0054633) as a biomarker for pre-diabetes and T2DM. Subjects & Methods: A total number of 149 subjects, selected from endocrinology unit of Internal Medicine Department, and Munshaat Sultan Family Medicine Clinic Menoufia university hospital, classified into three groups : group I were 55 T2DM patients, group II were 44 pre-diabetics and group III including 50 healthy subjects. Expression profile of circRNA (0054633) in plasma of the studied subjects was analyzed by quantitative real- time polymerase chain reaction (Q-PCR). Results: CircRNA (0054633) expression was significantly increased gradually from controls to the pre-diabetes group to the T2DM group (p<0.05).The sensitivity of CircRNA (0054633) expression for prediction of diabetes and prediabetes at cutoff point (2.95 ,1.95) was (92.7%, 77.3%) respectivily and the specificity was (98% and 98.6%) respectivily. The most significant pedictors for pre-diabetes were CircRNA (0054633) expression and BMI, OR (59.8, 24.9 respectivily). Conclusion: Plasma CircRNA (0054633) expression could be considered as a predictive and diagnostic biomarker for pre-diabetes and T2DM. | ||||
| Keywords | ||||
| Circular RNA; Pre-diabetes; T2DM | ||||
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