Possible Protective Role of Erythropoietin in Diabetic Cardiomyopathy in Rats | ||||
| Bulletin of Egyptian Society for Physiological Sciences | ||||
| Article 1, Volume 37, Issue 2, December 2017, Page 92-109 PDF (1.61 MB) | ||||
| Document Type: Original Article | ||||
| DOI: 10.21608/besps.2017.8231 | ||||
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| Authors | ||||
Mohammed R. Rabei1; Hanaa A. Abd-Elmoniem2; Mohammed E. Sarhan2; Nisreen M. Abo-Elmaaty  1; Lashin S. Ali2
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| 1Department of Medical Physiology,Faculty of Medicine,Mansoura University, Mansoura, Egypt. | ||||
| 2Department of Medical Physiology, Faculty of Medicine,Mansoura University, Mansoura, Egypt. | ||||
| Abstract | ||||
| There are controversial experimental works about the way of EPO in protection against DCM; whether EPO action is through making better metabolic condition and glycemic control or the antioxidant and antiapoptotic effect. So ,the aim of this study was to evaluate the modulating effects of EPO on some biochemical markers in diabetic cardiomyopathy. Fifty male Sprague Dawley rats were divided into five groups of 10rats each. The rats of group Ⅰ served as normal control. Group Ⅱ received HFD for 1month and STZ in a concentration of 35mg/kg intraperitoneal. Group Ⅲ:(Diab + Gliben) diabetic rats treated with glibenclamide, Gliben was given at a dose (0.6 mg/kg orally) for 4 weeks, Group Ⅳ: Diabetic rats treated with EPO; twice a week 4 weeks' duration. (1,000 IU/kg) and Group Ⅴ: diabetic rats treated by both (EPO + Gliben) with the same previous doses for 4 weeks' duration. At the end of experiment serum samples and cardiac tissues were obtained from the sacrificed rats. Cardiac enzymes, blood glucose levels, serum insulin level and oxidative stress markers were estimated. Diabetic cardiomyopathy changes were confirmed by electrophysiological and histopathological results as well as increased serum activity o f cardiac enzymes and high blood glucose levels. EPO exhibited myocardial protection in rats with DCM, at least in part through antioxidant effect and better glycemic control. However, this study was based on animal models in vivo, and detailed molecular mechanisms should be further investigated using in vitro studies. Clinical trials may also be considered to validate the results in humans, following further pre-clinical studies. | ||||
| Keywords | ||||
| Erythropoietin (EPO); Diabetic cardiomyopathy; Glibenclamide; Oxidative Stress; High Fat Diet (HFD) | ||||
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