Antiproliferative Effect of Green Tea and Grape Seed Mixture Extracts Combined with γ-irradiation on the Growth of Myeloma Cells
El-Shershaby, H., Eldesouky, W., Ebeid, N. (2018). Antiproliferative Effect of Green Tea and Grape Seed Mixture Extracts Combined with γ-irradiation on the Growth of Myeloma Cells. EKB Journal Management System, 51(3), 139-151. doi: 10.21608/ajnsa.2018.2329.1023
Hanan El-Shershaby; Wael Eldesouky; Nahed Ebeid. "Antiproliferative Effect of Green Tea and Grape Seed Mixture Extracts Combined with γ-irradiation on the Growth of Myeloma Cells". EKB Journal Management System, 51, 3, 2018, 139-151. doi: 10.21608/ajnsa.2018.2329.1023
El-Shershaby, H., Eldesouky, W., Ebeid, N. (2018). 'Antiproliferative Effect of Green Tea and Grape Seed Mixture Extracts Combined with γ-irradiation on the Growth of Myeloma Cells', EKB Journal Management System, 51(3), pp. 139-151. doi: 10.21608/ajnsa.2018.2329.1023
El-Shershaby, H., Eldesouky, W., Ebeid, N. Antiproliferative Effect of Green Tea and Grape Seed Mixture Extracts Combined with γ-irradiation on the Growth of Myeloma Cells. EKB Journal Management System, 2018; 51(3): 139-151. doi: 10.21608/ajnsa.2018.2329.1023

Antiproliferative Effect of Green Tea and Grape Seed Mixture Extracts Combined with γ-irradiation on the Growth of Myeloma Cells

Arab Journal of Nuclear Sciences and Applications
Article 15, Volume 51, Issue 3, July 2018, Page 139-151  XML PDF (846.93 K)
Document Type: Original Article
DOI: 10.21608/ajnsa.2018.2329.1023
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Authors
Hanan El-Shershaby1; Wael Eldesouky email 2; Nahed Ebeid1
1Labeled Compounds Dept., Hot Laboratories Center, Atomic Energy Authority, Egypt
2Radiation Protection dept., Hot Laboratories Center, Atomic Energy Authority
Abstract
Purpose: The purpose of this work is to study the effect of green tea and grape seed extracts mixture (GTE-GSE mix) and γ-irradiation or their combination to enhance the antiproliferative effect on the growth of myeloma cells.
Method: Myeloma cell line SP2/OR was cultured in RPMI-1640 medium; total count and viability % were daily calculated. Myeloma cells (1 X 106/ml) were treated with GTE-GSE mix concentrations (100 - 1000 ng/ml), γ-irradiation doses (0.5 - 10 Gy) and their combination for 6 days. Evaluation of Caspase 8 and 9 activities were measured. Cell culture media and GTE-GSE mix activity was determined by DPPH method.
Results: The most effective dose of GTE-GSE mix on myeloma cells growth inhibition and viability % was 1000 ng/ml, while the effective dose of γ-irradiation was 8 Gy where viability percentage was zero. The obtained results indicated that treatment with GTE-GSE mix plus γ-irradiation combination (700 ng/ml + 6 Gy) resulted to zero% growth inhibition at 4th day. Treatment with combination dose induced activation of caspase 8 and caspase 9 levels more than GTE-GSE mix and γ-irradiation alone. Treatment with GTE-GSE mix and γ-irradiation individually resulted in significant reduction in DPPH activity to 62% while in combination treatment was 73.5%.
Conclusion: Treatment with GTE-GSE mix plus γ-irradiation (700 ng/ml + 6 Gy) combination resulted in marked increase of growth inhibition levels, apoptosis and cancer cells regression when compared with each agent alone. GTE-GSE mix plus γ-irradiation combination had an antiproliferative effect on the growth of myeloma cells.
Keywords
Antiproliferative; Myeloma cells; Green tea extract; Grape seed extract; γ-irradiation
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