ALTERATIONS IN P53 GENE, TESTICULAR AND HEPATIC TISSUES OF ALBINO RATS DUE TO PROFENOFOS ADMINISTRATION: A POSSIBLE PROTECTIVE EFFECT OF VITAMIN C.
Dessouki, A., Hassan, A., Ali, S., Loutfy, N. (2012). ALTERATIONS IN P53 GENE, TESTICULAR AND HEPATIC TISSUES OF ALBINO RATS DUE TO PROFENOFOS ADMINISTRATION: A POSSIBLE PROTECTIVE EFFECT OF VITAMIN C.. EKB Journal Management System, 3(5), 415-433. doi: 10.21608/jppp.2012.83785
Amina Dessouki; Abeer Hassan; Saadia Ali; Naglaa Loutfy. "ALTERATIONS IN P53 GENE, TESTICULAR AND HEPATIC TISSUES OF ALBINO RATS DUE TO PROFENOFOS ADMINISTRATION: A POSSIBLE PROTECTIVE EFFECT OF VITAMIN C.". EKB Journal Management System, 3, 5, 2012, 415-433. doi: 10.21608/jppp.2012.83785
Dessouki, A., Hassan, A., Ali, S., Loutfy, N. (2012). 'ALTERATIONS IN P53 GENE, TESTICULAR AND HEPATIC TISSUES OF ALBINO RATS DUE TO PROFENOFOS ADMINISTRATION: A POSSIBLE PROTECTIVE EFFECT OF VITAMIN C.', EKB Journal Management System, 3(5), pp. 415-433. doi: 10.21608/jppp.2012.83785
Dessouki, A., Hassan, A., Ali, S., Loutfy, N. ALTERATIONS IN P53 GENE, TESTICULAR AND HEPATIC TISSUES OF ALBINO RATS DUE TO PROFENOFOS ADMINISTRATION: A POSSIBLE PROTECTIVE EFFECT OF VITAMIN C.. EKB Journal Management System, 2012; 3(5): 415-433. doi: 10.21608/jppp.2012.83785

ALTERATIONS IN P53 GENE, TESTICULAR AND HEPATIC TISSUES OF ALBINO RATS DUE TO PROFENOFOS ADMINISTRATION: A POSSIBLE PROTECTIVE EFFECT OF VITAMIN C.

Journal of Plant Protection and Pathology
Article 3, Volume 3, Issue 5, May 2012, Page 415-433  XML PDF (602.83 K)
Document Type: Original Article
DOI: 10.21608/jppp.2012.83785
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Authors
Amina Dessouki1; Abeer Hassan2; Saadia Ali3; Naglaa Loutfy4
1Department of Pathology Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522 Egyp
2Biochemistry and Physiology Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522 Egypt
3Biochemistry and Physiology , Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522 Egypt
4Department of Plant Protection, Faculty of Agriculture, Suez Canal University, Ismailia, 41522 Egypt.
Abstract
The effects of profenofos on rat testis and liver was evaluated when applied for 10 weeks, either alone or mixed with vitamin C.  Sperm counts, motility, morphology, reduced glutathione, catalase, superoxide dismutase, malodialdehyde, testosterone level, RT-PCR SSCP of gene P53 were studies. Significant decrease in sperm motility and survival were observed in treated rats. Serum testosterone decreased after exposure for 5 and 10 weeks. MDA content in serum, liver and testis showed significant increase. The activity of hepatic SOD increased, while CAT activity was not changed after 5 weeks exposure. After 10 weeks exposure, the activity of SOD and CAT in both liver and testis, were lower than the control group. At the end of the two exposure periods, GSH levels in both tissues were significantly higher in profenofos treated groups compared to control group.  Results of 10 weeks exposure using RT- PCR SSCP analysis suggested profenofos induction of genotoxicity.  
Keywords
Profenofos; rats; p53 gene; SSCP; SOD; CAT, GSH, histopathology; spermogram
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