PHYTOCHEMICAL AND MOLECULAR STUDIES ON NARCISSUS IN EGYPT
Abd El-Ghany, M., Mohamed, F., El-Ashry, Z., Abd El-Rahman, H. (2012). PHYTOCHEMICAL AND MOLECULAR STUDIES ON NARCISSUS IN EGYPT. EKB Journal Management System, 3(3), 545-556. doi: 10.21608/jpp.2012.84193
M. N. Abd El-Ghany; Fawzyia I. Mohamed; Zeinab M. El-Ashry; H. M. Abd El-Rahman. "PHYTOCHEMICAL AND MOLECULAR STUDIES ON NARCISSUS IN EGYPT". EKB Journal Management System, 3, 3, 2012, 545-556. doi: 10.21608/jpp.2012.84193
Abd El-Ghany, M., Mohamed, F., El-Ashry, Z., Abd El-Rahman, H. (2012). 'PHYTOCHEMICAL AND MOLECULAR STUDIES ON NARCISSUS IN EGYPT', EKB Journal Management System, 3(3), pp. 545-556. doi: 10.21608/jpp.2012.84193
Abd El-Ghany, M., Mohamed, F., El-Ashry, Z., Abd El-Rahman, H. PHYTOCHEMICAL AND MOLECULAR STUDIES ON NARCISSUS IN EGYPT. EKB Journal Management System, 2012; 3(3): 545-556. doi: 10.21608/jpp.2012.84193

PHYTOCHEMICAL AND MOLECULAR STUDIES ON NARCISSUS IN EGYPT

Journal of Plant Production
Article 10, Volume 3, Issue 3, March 2012, Page 545-556  XML PDF (606.21 K)
Document Type: Original Article
DOI: 10.21608/jpp.2012.84193
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Authors
M. N. Abd El-Ghany1; Fawzyia I. Mohamed2; Zeinab M. El-Ashry2; H. M. Abd El-Rahman2
1Botany Department, Faculty of Science, Mansoura University
2Department of Genetics and Cytology, National Research Center, Dokki, Cairo, Egypt.
Abstract
This study was carried out in NationalResearchCenter in Dokki, Giza, Egypt in collaboration with Botany department, Faculty of Science, MansouraUniversity during 2007-2009 seasons.  This study first, aims to find Egyptian wild narcissus bulbs for scientific records and genetic fingerprinting as an Egyptian species. Unfortunately the bulbs almost disappeared from about two decades under the pressure of tourism and ordinary cultivation in north coast of the country.   Hopeless looking for any evidence it may be still found, but our search in many areas in Egypt fails to find any bulb.  And so, the study continues based on another two species of narcissus foreign one Narcissus tazetta cultivated for many years in Egypt and a commercial variety from flowers producer.  The other aim of our study was to carry out phytochemical screening to estimate the natural components of Narcissus tazetta plants and data showed that many constituents were found in the form of alkaloids including flavonoids, tannins, sterols and triperpenes.  Finally, molecular studies using RAPD-PCR were carried out to fingerprint the two species of narcissus using ten 10-mer primers, indicating that unique fingerprinting bands as produced by primers number 1, 2 and 3 could clearly discriminate between the two species for example, fragments with approximately molecular size of 2764 bp, 2344 bp, 1114 bp, 978 bp and 792 bp were detected in Narcissus tazetta profile while, fragments with approximately molecular size of 1787 bp, 1996 bp, 1592 bp and 583 bp were detected in commercial narcissus profile.
Keywords
Narcissus; RAPD-PCR; Phytochemical screening; secondary metabolites
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